Generation of non-integrated induced pluripotent stem cells from a 59-year-old female with multiple endocrine neoplasia type 1 syndrome

Dongsheng Guo; Feima Wu; Haikun Liu; Ge Gao; Shanglong Kou; Fan Yang; Nasir Abbas; Tiancheng Zhou; Xiujuan Cai; Hui Zhang; Dajiang Qin; Jialiang Li; Kecheng Xu; Yin xiong Li

doi:10.1016/j.scr.2016.12.009

Generation of non-integrated induced pluripotent stem cells from a 59-year-old female with multiple endocrine neoplasia type 1 syndrome

Dongsheng Guo, Feima Wu, Haikun Liu, Ge Gao, Shanglong Kou, Fan Yang, Nasir Abbas, Tiancheng Zhou, Xiujuan Cai, Hui Zhang, Dajiang Qin, Jialiang Li, Kecheng Xu, Yin xiong Li^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Urine resource cells were collected from a 59-year-old female patient with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids carrying Oct4, Sox2, Klf4 and miR-302-367. The patient sustained a heterozygous G > T transition mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on the obtained iPSC lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression, as well as their abilities for differentiating into three germ layers. This cell line provides an ideal model for studying MEN1.

Original language	English
Pages (from-to)	64-66
Number of pages	3
Journal	Stem Cell Research
Volume	18
DOIs	https://doi.org/10.1016/j.scr.2016.12.009
Publication status	Published - Jan 2017
Externally published	Yes

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title = "Generation of non-integrated induced pluripotent stem cells from a 59-year-old female with multiple endocrine neoplasia type 1 syndrome",

abstract = "Urine resource cells were collected from a 59-year-old female patient with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids carrying Oct4, Sox2, Klf4 and miR-302-367. The patient sustained a heterozygous G > T transition mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on the obtained iPSC lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression, as well as their abilities for differentiating into three germ layers. This cell line provides an ideal model for studying MEN1.",

author = "Dongsheng Guo and Feima Wu and Haikun Liu and Ge Gao and Shanglong Kou and Fan Yang and Nasir Abbas and Tiancheng Zhou and Xiujuan Cai and Hui Zhang and Dajiang Qin and Jialiang Li and Kecheng Xu and Li, {Yin xiong}",

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year = "2017",

month = jan,

doi = "10.1016/j.scr.2016.12.009",

language = "English",

volume = "18",

pages = "64--66",

journal = "Stem Cell Research",

issn = "1873-5061",

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TY - JOUR

T1 - Generation of non-integrated induced pluripotent stem cells from a 59-year-old female with multiple endocrine neoplasia type 1 syndrome

AU - Guo, Dongsheng

AU - Wu, Feima

AU - Liu, Haikun

AU - Gao, Ge

AU - Kou, Shanglong

AU - Yang, Fan

AU - Abbas, Nasir

AU - Zhou, Tiancheng

AU - Cai, Xiujuan

AU - Zhang, Hui

AU - Qin, Dajiang

AU - Li, Jialiang

AU - Xu, Kecheng

AU - Li, Yin xiong

PY - 2017/1

Y1 - 2017/1

N2 - Urine resource cells were collected from a 59-year-old female patient with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids carrying Oct4, Sox2, Klf4 and miR-302-367. The patient sustained a heterozygous G > T transition mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on the obtained iPSC lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression, as well as their abilities for differentiating into three germ layers. This cell line provides an ideal model for studying MEN1.

AB - Urine resource cells were collected from a 59-year-old female patient with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids carrying Oct4, Sox2, Klf4 and miR-302-367. The patient sustained a heterozygous G > T transition mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on the obtained iPSC lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression, as well as their abilities for differentiating into three germ layers. This cell line provides an ideal model for studying MEN1.

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DO - 10.1016/j.scr.2016.12.009

M3 - Article

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AN - SCOPUS:85008239806

SN - 1873-5061

VL - 18

SP - 64

EP - 66

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of non-integrated induced pluripotent stem cells from a 59-year-old female with multiple endocrine neoplasia type 1 syndrome

Abstract

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