Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

Fang Yuan; Dongsheng Guo; Yanli Liu; Yingying Xu; Ge Gao; Yuhang Wu; Fan Yang; Xinrong Ke; Keyu Lai; Hongcheng Wei; Yin Xiong Li

doi:10.1016/j.scr.2017.11.007

Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

Fang Yuan, Dongsheng Guo, Yanli Liu, Yingying Xu, Ge Gao, Yuhang Wu, Fan Yang, Xinrong Ke, Keyu Lai, Hongcheng Wei^*, Yin Xiong Li

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.

Original language	English
Pages (from-to)	67-71
Number of pages	5
Journal	Stem Cell Research
Volume	26
DOIs	https://doi.org/10.1016/j.scr.2017.11.007
Publication status	Published - Jan 2018
Externally published	Yes

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10.1016/j.scr.2017.11.007

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title = "Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9",

abstract = "The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.",

author = "Fang Yuan and Dongsheng Guo and Yanli Liu and Yingying Xu and Ge Gao and Yuhang Wu and Fan Yang and Xinrong Ke and Keyu Lai and Hongcheng Wei and Li, \{Yin Xiong\}",

note = "Publisher Copyright: {\textcopyright} 2017",

year = "2018",

month = jan,

doi = "10.1016/j.scr.2017.11.007",

language = "English",

volume = "26",

pages = "67--71",

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T1 - Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

AU - Yuan, Fang

AU - Guo, Dongsheng

AU - Liu, Yanli

AU - Xu, Yingying

AU - Gao, Ge

AU - Wu, Yuhang

AU - Yang, Fan

AU - Ke, Xinrong

AU - Lai, Keyu

AU - Wei, Hongcheng

AU - Li, Yin Xiong

PY - 2018/1

Y1 - 2018/1

N2 - The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.

AB - The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.

UR - https://www.scopus.com/pages/publications/85037986258

U2 - 10.1016/j.scr.2017.11.007

DO - 10.1016/j.scr.2017.11.007

M3 - Article

C2 - 29247816

AN - SCOPUS:85037986258

SN - 1873-5061

VL - 26

SP - 67

EP - 71

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

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