Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9

Yingying Xu; Yuhang Wu; Dongsheng Guo; Ge Gao; Keyu Lai; Fan Yang; Kepin Wang; Han Wu; Liangxue Lai; Jialiang Li; Kecheng Xu; Yin xiong Li

doi:10.1016/j.scr.2017.05.006

Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9

Yingying Xu, Yuhang Wu, Dongsheng Guo, Ge Gao, Keyu Lai, Fan Yang, Kepin Wang, Han Wu, Liangxue Lai, Jialiang Li, Kecheng Xu^*, Yin xiong Li

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37 bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro.

Original language	English
Pages (from-to)	29-32
Number of pages	4
Journal	Stem Cell Research
Volume	22
DOIs	https://doi.org/10.1016/j.scr.2017.05.006
Publication status	Published - Jul 2017
Externally published	Yes

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title = "Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9",

abstract = "The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37 bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro.",

author = "Yingying Xu and Yuhang Wu and Dongsheng Guo and Ge Gao and Keyu Lai and Fan Yang and Kepin Wang and Han Wu and Liangxue Lai and Jialiang Li and Kecheng Xu and Li, {Yin xiong}",

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year = "2017",

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doi = "10.1016/j.scr.2017.05.006",

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T1 - Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9

AU - Xu, Yingying

AU - Wu, Yuhang

AU - Guo, Dongsheng

AU - Gao, Ge

AU - Lai, Keyu

AU - Yang, Fan

AU - Wang, Kepin

AU - Wu, Han

AU - Lai, Liangxue

AU - Li, Jialiang

AU - Xu, Kecheng

AU - Li, Yin xiong

PY - 2017/7

Y1 - 2017/7

N2 - The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37 bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro.

AB - The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37 bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro.

UR - http://www.scopus.com/inward/record.url?scp=85019726600&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2017.05.006

DO - 10.1016/j.scr.2017.05.006

M3 - Article

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AN - SCOPUS:85019726600

SN - 1873-5061

VL - 22

SP - 29

EP - 32

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9

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