Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing

Feima Wu; Ge Gao; Tingcai Pan; Zhen Yang; Guosheng Xu; Nasir Abbas; Yanli Liu; Yan Chen; Shenglin Tan; Kai You; Xinrong Ke; Yuanqi Zhuang; Xianhua Lin; Fan Yang; Yin xiong Li

doi:10.1016/j.scr.2017.12.002

Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing

Feima Wu, Ge Gao, Tingcai Pan, Zhen Yang, Guosheng Xu, Nasir Abbas, Yanli Liu, Yan Chen, Shenglin Tan, Kai You, Xinrong Ke, Yuanqi Zhuang, Xianhua Lin, Fan Yang, Yin xiong Li^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40 bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers.

Original language	English
Pages (from-to)	5-9
Number of pages	5
Journal	Stem Cell Research
Volume	27
DOIs	https://doi.org/10.1016/j.scr.2017.12.002
Publication status	Published - Mar 2018
Externally published	Yes

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title = "Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing",

abstract = "The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40 bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers.",

author = "Feima Wu and Ge Gao and Tingcai Pan and Zhen Yang and Guosheng Xu and Nasir Abbas and Yanli Liu and Yan Chen and Shenglin Tan and Kai You and Xinrong Ke and Yuanqi Zhuang and Xianhua Lin and Fan Yang and Li, {Yin xiong}",

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year = "2018",

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doi = "10.1016/j.scr.2017.12.002",

language = "English",

volume = "27",

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T1 - Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing

AU - Wu, Feima

AU - Gao, Ge

AU - Pan, Tingcai

AU - Yang, Zhen

AU - Xu, Guosheng

AU - Abbas, Nasir

AU - Liu, Yanli

AU - Chen, Yan

AU - Tan, Shenglin

AU - You, Kai

AU - Ke, Xinrong

AU - Zhuang, Yuanqi

AU - Lin, Xianhua

AU - Yang, Fan

AU - Li, Yin xiong

PY - 2018/3

Y1 - 2018/3

N2 - The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40 bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers.

AB - The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40 bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers.

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U2 - 10.1016/j.scr.2017.12.002

DO - 10.1016/j.scr.2017.12.002

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AN - SCOPUS:85039718082

SN - 1873-5061

VL - 27

SP - 5

EP - 9

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing

Abstract

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