Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9

Fang Yuan; Dongsheng Guo; Ge Gao; Yanli Liu; Yingying Xu; Yuhang Wu; Fan Yang; Xinrong Ke; Keyu Lai; Liangqing Hong; Yin xiong Li

doi:10.1016/j.scr.2017.08.016

Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9

Fang Yuan, Dongsheng Guo, Ge Gao, Yanli Liu, Yingying Xu, Yuhang Wu, Fan Yang, Xinrong Ke, Keyu Lai, Liangqing Hong^*, Yin xiong Li

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The ATP-sensitive potassium channel is an octameric complex, and one of its subunits, namely Kir6.2, is encoded by the KCNJ11 gene. Mutations in KCNJ11 result in hyperinsulinism or diabetes mellitus, associated with abnormal insulin secretion. Here, using CRISPR/Cas9 editing, we established a homozygous mutant KCNJ11 cell line, WAe001-A-12, which was generated by a 62-bp deletion in the coding sequence of the human embryonic stem cell line H1. It was confirmed that this deletion in the KCNJ11 gene did not affect the protein expression levels of key pluripotent factors. Additionally, normal karyotype and differentiation potency were observed for the cell line.

Original language	English
Pages (from-to)	89-93
Number of pages	5
Journal	Stem Cell Research
Volume	24
DOIs	https://doi.org/10.1016/j.scr.2017.08.016
Publication status	Published - Oct 2017
Externally published	Yes

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title = "Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9",

abstract = "The ATP-sensitive potassium channel is an octameric complex, and one of its subunits, namely Kir6.2, is encoded by the KCNJ11 gene. Mutations in KCNJ11 result in hyperinsulinism or diabetes mellitus, associated with abnormal insulin secretion. Here, using CRISPR/Cas9 editing, we established a homozygous mutant KCNJ11 cell line, WAe001-A-12, which was generated by a 62-bp deletion in the coding sequence of the human embryonic stem cell line H1. It was confirmed that this deletion in the KCNJ11 gene did not affect the protein expression levels of key pluripotent factors. Additionally, normal karyotype and differentiation potency were observed for the cell line.",

author = "Fang Yuan and Dongsheng Guo and Ge Gao and Yanli Liu and Yingying Xu and Yuhang Wu and Fan Yang and Xinrong Ke and Keyu Lai and Liangqing Hong and Li, {Yin xiong}",

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doi = "10.1016/j.scr.2017.08.016",

language = "English",

volume = "24",

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T1 - Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9

AU - Yuan, Fang

AU - Guo, Dongsheng

AU - Gao, Ge

AU - Liu, Yanli

AU - Xu, Yingying

AU - Wu, Yuhang

AU - Yang, Fan

AU - Ke, Xinrong

AU - Lai, Keyu

AU - Hong, Liangqing

AU - Li, Yin xiong

PY - 2017/10

Y1 - 2017/10

N2 - The ATP-sensitive potassium channel is an octameric complex, and one of its subunits, namely Kir6.2, is encoded by the KCNJ11 gene. Mutations in KCNJ11 result in hyperinsulinism or diabetes mellitus, associated with abnormal insulin secretion. Here, using CRISPR/Cas9 editing, we established a homozygous mutant KCNJ11 cell line, WAe001-A-12, which was generated by a 62-bp deletion in the coding sequence of the human embryonic stem cell line H1. It was confirmed that this deletion in the KCNJ11 gene did not affect the protein expression levels of key pluripotent factors. Additionally, normal karyotype and differentiation potency were observed for the cell line.

AB - The ATP-sensitive potassium channel is an octameric complex, and one of its subunits, namely Kir6.2, is encoded by the KCNJ11 gene. Mutations in KCNJ11 result in hyperinsulinism or diabetes mellitus, associated with abnormal insulin secretion. Here, using CRISPR/Cas9 editing, we established a homozygous mutant KCNJ11 cell line, WAe001-A-12, which was generated by a 62-bp deletion in the coding sequence of the human embryonic stem cell line H1. It was confirmed that this deletion in the KCNJ11 gene did not affect the protein expression levels of key pluripotent factors. Additionally, normal karyotype and differentiation potency were observed for the cell line.

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DO - 10.1016/j.scr.2017.08.016

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EP - 93

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9

Abstract

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