Friedmanniella flava sp. nov., a soil actinomycete

A novel actinomycete, strain W6^T, was isolated from a soil sample of Yunnan Province, China. The bacterium was aerobic, non-motile, non-spore-forming and Gram-stain-positive. Genetic, phenotypic and chemical properties of the isolate were studied. 16S rRNA gene sequence data suggested that the novel isolate belonged to the genus Friedmanniella and shared 98.6 % sequence similarity with Friedmanniella antarctica DSM 11053^T and Friedmanniella okinawensis DSM 21744^T, the most closely related species. The cell-wall peptidoglycan contained LL-diaminopimelic acid, and mycolic acids were absent. The main menaquinone was MK-9(H₄) and the predominant fatty acids were anteiso-C_15:0 and iso-C_15:0. The phospholipid profile contained phosphatidylglycerol, phosphatidylinositol, phosphatidylcholine and diphosphatidylglycerol. The DNA G+C content of strain W6^T was 72 mol%. Strain W6^T showed 30.0 % and 28.5 % DNA-DNA relatedness, respectively, to F. antarctica DSM 11053^T and F. okinawensis DSM 21744^T. The combined genotypic and phenotypic data showed that strain W6^T should be assigned to the genus Friedmanniella as a representative of a novel species, for which the name Friedmanniella flava sp. nov. is proposed. The type strain is W6^T (= CGMCC 4.6856^T = JCM 17701^T).