FRET effect on DNA fluorescent molecular probe by graphene oxide

Cong Xiao Zhang; Xue Fei Lü; Hong Qing; Yu Lin Deng

doi:10.15918/j.tbit1001-0645.2015.supplement1.027

FRET effect on DNA fluorescent molecular probe by graphene oxide

Cong Xiao Zhang
, Xue Fei Lü
, Hong Qing
, Yu Lin Deng^*

^*Corresponding author for this work

School of Medical Science and Engineering

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Fluorescence resonance energy transfer (FRET) effect of graphene oxide on DNA fluorescent molecular probe is a very important factor of DNA the biosensor, which is related to the detection sensitivity of graphene oxide based DNA biosensors. The effects of the concentration of graphene oxide, the reaction temperature, and the length of DNA on FRET were investigated. The results show that the concentration of graphene oxide, the reaction temperature, and the length of DNA play significant roles on FRET effects. The fluorescence quenching efficiency of DNA molecule probe was improved with the increasing of graphene oxide concentration and the reaction temperature, with the decreasing of the length of DNA. Therefore, in order to get the excellent biosensor, the concentration of graphene oxide, the reaction temperature, and the length of DNA should be taken into account in the design of graphene oxide DNA biosensor.

Original language	English
Pages (from-to)	123-127
Number of pages	5
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	35
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.027
Publication status	Published - 1 Jul 2015

Keywords

Fluorescence resonance energy transfer (FRET)
Graphene oxide
Molecular probe

Access to Document

10.15918/j.tbit1001-0645.2015.supplement1.027

Cite this

@article{7c1cf68847d44389a41084061482a6e6,

title = "FRET effect on DNA fluorescent molecular probe by graphene oxide",

abstract = "Fluorescence resonance energy transfer (FRET) effect of graphene oxide on DNA fluorescent molecular probe is a very important factor of DNA the biosensor, which is related to the detection sensitivity of graphene oxide based DNA biosensors. The effects of the concentration of graphene oxide, the reaction temperature, and the length of DNA on FRET were investigated. The results show that the concentration of graphene oxide, the reaction temperature, and the length of DNA play significant roles on FRET effects. The fluorescence quenching efficiency of DNA molecule probe was improved with the increasing of graphene oxide concentration and the reaction temperature, with the decreasing of the length of DNA. Therefore, in order to get the excellent biosensor, the concentration of graphene oxide, the reaction temperature, and the length of DNA should be taken into account in the design of graphene oxide DNA biosensor.",

keywords = "Fluorescence resonance energy transfer (FRET), Graphene oxide, Molecular probe",

author = "Zhang, \{Cong Xiao\} and L{\"u}, \{Xue Fei\} and Hong Qing and Deng, \{Yu Lin\}",

year = "2015",

month = jul,

day = "1",

doi = "10.15918/j.tbit1001-0645.2015.supplement1.027",

language = "English",

volume = "35",

pages = "123--127",

journal = "Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology",

issn = "1001-0645",

publisher = "Beijing Institute of Technology",

}

TY - JOUR

T1 - FRET effect on DNA fluorescent molecular probe by graphene oxide

AU - Zhang, Cong Xiao

AU - Lü, Xue Fei

AU - Qing, Hong

AU - Deng, Yu Lin

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Fluorescence resonance energy transfer (FRET) effect of graphene oxide on DNA fluorescent molecular probe is a very important factor of DNA the biosensor, which is related to the detection sensitivity of graphene oxide based DNA biosensors. The effects of the concentration of graphene oxide, the reaction temperature, and the length of DNA on FRET were investigated. The results show that the concentration of graphene oxide, the reaction temperature, and the length of DNA play significant roles on FRET effects. The fluorescence quenching efficiency of DNA molecule probe was improved with the increasing of graphene oxide concentration and the reaction temperature, with the decreasing of the length of DNA. Therefore, in order to get the excellent biosensor, the concentration of graphene oxide, the reaction temperature, and the length of DNA should be taken into account in the design of graphene oxide DNA biosensor.

AB - Fluorescence resonance energy transfer (FRET) effect of graphene oxide on DNA fluorescent molecular probe is a very important factor of DNA the biosensor, which is related to the detection sensitivity of graphene oxide based DNA biosensors. The effects of the concentration of graphene oxide, the reaction temperature, and the length of DNA on FRET were investigated. The results show that the concentration of graphene oxide, the reaction temperature, and the length of DNA play significant roles on FRET effects. The fluorescence quenching efficiency of DNA molecule probe was improved with the increasing of graphene oxide concentration and the reaction temperature, with the decreasing of the length of DNA. Therefore, in order to get the excellent biosensor, the concentration of graphene oxide, the reaction temperature, and the length of DNA should be taken into account in the design of graphene oxide DNA biosensor.

KW - Fluorescence resonance energy transfer (FRET)

KW - Graphene oxide

KW - Molecular probe

UR - https://www.scopus.com/pages/publications/84943400543

U2 - 10.15918/j.tbit1001-0645.2015.supplement1.027

DO - 10.15918/j.tbit1001-0645.2015.supplement1.027

M3 - Article

AN - SCOPUS:84943400543

SN - 1001-0645

VL - 35

SP - 123

EP - 127

JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

FRET effect on DNA fluorescent molecular probe by graphene oxide

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this