TY - JOUR
T1 - Fluorescent carbonaceous nanodots for noninvasive glioma imaging after angiopep-2 decoration
AU - Ruan, Shaobo
AU - Qian, Jun
AU - Shen, Shun
AU - Chen, Jiantao
AU - Zhu, Jianhua
AU - Jiang, Xinguo
AU - He, Qin
AU - Yang, Wuli
AU - Gao, Huile
N1 - Publisher Copyright:
© 2014 American Chemical Society.
PY - 2014/12/17
Y1 - 2014/12/17
N2 - (Chemical Equation Presented) Fluorescent carbonaceous nanodots (CDs) have attracted much attention due to their unique properties. However, their application in noninvasive imaging of diseased tissues was restricted by the short excitation/emission wavelengths and the low diseased tissue accumulation efficiency. In this study, CDs were prepared from glucose and glutamic acid with a particle size of 4 nm. Obvious emission could be observed at 600 to 700 nm when CDs were excited at around 500 nm. This property enabled CDs with capacity for deep tissue imaging with low background adsorption. Angiopep-2, a ligand which could target glioma cells, was anchored onto CDs after PEGylation. The product, An-PEG-CDs, could target C6 glioma cells with higher intensity than PEGylated CDs (PEG-CDs), and endosomes were involved in the uptake process. In vivo, An-PEG-CDs could accumulate in the glioma site at higher intensity, as the glioma/normal brain ratio for An-PEG-CDs was 1.73. The targeting effect of An-PEG-CDs was further demonstrated by receptor staining, which showed An-PEG-CDs colocalized well with the receptors expressed in glioma. In conclusion, An-PEG-CDs could be successfully used for noninvasive glioma imaging.
AB - (Chemical Equation Presented) Fluorescent carbonaceous nanodots (CDs) have attracted much attention due to their unique properties. However, their application in noninvasive imaging of diseased tissues was restricted by the short excitation/emission wavelengths and the low diseased tissue accumulation efficiency. In this study, CDs were prepared from glucose and glutamic acid with a particle size of 4 nm. Obvious emission could be observed at 600 to 700 nm when CDs were excited at around 500 nm. This property enabled CDs with capacity for deep tissue imaging with low background adsorption. Angiopep-2, a ligand which could target glioma cells, was anchored onto CDs after PEGylation. The product, An-PEG-CDs, could target C6 glioma cells with higher intensity than PEGylated CDs (PEG-CDs), and endosomes were involved in the uptake process. In vivo, An-PEG-CDs could accumulate in the glioma site at higher intensity, as the glioma/normal brain ratio for An-PEG-CDs was 1.73. The targeting effect of An-PEG-CDs was further demonstrated by receptor staining, which showed An-PEG-CDs colocalized well with the receptors expressed in glioma. In conclusion, An-PEG-CDs could be successfully used for noninvasive glioma imaging.
UR - https://www.scopus.com/pages/publications/84918593988
U2 - 10.1021/bc500474p
DO - 10.1021/bc500474p
M3 - Article
C2 - 25387274
AN - SCOPUS:84918593988
SN - 1043-1802
VL - 25
SP - 2252
EP - 2259
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
IS - 12
ER -