TY - JOUR
T1 - Fluorescent AIE-Active Materials for Two-Photon Bioimaging Applications
AU - Lu, Qing
AU - Wu, Cheng Juan
AU - Liu, Zhiqiang
AU - Niu, Guangle
AU - Yu, Xiaoqiang
N1 - Publisher Copyright:
© Copyright © 2020 Lu, Wu, Liu, Niu and Yu.
PY - 2020/12/14
Y1 - 2020/12/14
N2 - Fluorescence imaging has been widely used as a powerful tool for in situ and real-time visualization of important analytes and biological events in live samples with remarkably high selectivity, sensitivity, and spatial resolution. Compared with one-photon fluorescence imaging, two-photon fluorescence imaging exhibits predominant advantages of minimal photodamage to samples, deep tissue penetration, and outstanding resolution. Recently, the aggregation-induced emission (AIE) materials have become a preferred choice in two-photon fluorescence biological imaging because of its unique bright fluorescence in solid and aggregate states and strong resistance to photobleaching. In this review, we will exclusively summarize the applications of AIE-active materials in two-photon fluorescence imaging with some representative examples from four aspects: fluorescence detection, in vitro cell imaging, ex vivo tissue imaging, and in vivo vascular imaging. In addition, the current challenges and future development directions of AIE-active materials for two-photon bioimaging are briefly discussed.
AB - Fluorescence imaging has been widely used as a powerful tool for in situ and real-time visualization of important analytes and biological events in live samples with remarkably high selectivity, sensitivity, and spatial resolution. Compared with one-photon fluorescence imaging, two-photon fluorescence imaging exhibits predominant advantages of minimal photodamage to samples, deep tissue penetration, and outstanding resolution. Recently, the aggregation-induced emission (AIE) materials have become a preferred choice in two-photon fluorescence biological imaging because of its unique bright fluorescence in solid and aggregate states and strong resistance to photobleaching. In this review, we will exclusively summarize the applications of AIE-active materials in two-photon fluorescence imaging with some representative examples from four aspects: fluorescence detection, in vitro cell imaging, ex vivo tissue imaging, and in vivo vascular imaging. In addition, the current challenges and future development directions of AIE-active materials for two-photon bioimaging are briefly discussed.
KW - aggregation-induced emission
KW - fluorescence imaging
KW - fluorescent detection
KW - fluorescent material
KW - two-photon
UR - http://www.scopus.com/inward/record.url?scp=85098284987&partnerID=8YFLogxK
U2 - 10.3389/fchem.2020.617463
DO - 10.3389/fchem.2020.617463
M3 - Review article
AN - SCOPUS:85098284987
SN - 2296-2646
VL - 8
JO - Frontiers in Chemistry
JF - Frontiers in Chemistry
M1 - 617463
ER -