Flavobacterium noncentrifugens sp. nov., a psychrotolerant bacterium isolated from glacier meltwater

A non-motile, Gram-stain-negative bacterium, designated R-HLS-17^T, was isolated from the meltwater of Hailuogou Glacier located in Sichuan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Flavobacterium, with the closest relatives being Flavobacterium antarcticum JCM 12383^T (95.5% 16S rRNA gene sequence similarity), F. omnivorum JCM 11313^T (95.0 %) and F. fryxellicola LMG 22022^T (95.2 %). Growth occurred at 0-29 ° C (optimum, 10-20 6C) and pH 6.0-8.5 (optimum, 7.0-8.0). The DNA G+C content was 46.5 mol%. The major cellular fatty acids were iso-C_{15: 0}, iso-C_{15: 1} G, summed feature 9 (comprising iso-C_{17: 1}ω9c and/or 10- methyl C_{16: 0}), iso-C_{17: 0} 3-OH and iso-C_{15: 0} 3-OH. The predominant menaquinone was MK-6. Based on the genotypic and phenotypic characteristics, we propose that strain R-HLS-17^T represents a novel species of the genus Flavobacterium, Flavobacterium noncentrifugens sp. nov. The type strain is R-HLS-17T (=CGMCC 1.10076^T=NBRC 108844^T).