FIS activates glnAp2 in Escherichia coli: Role of a DNA bend centered at -55, upstream of the transcription start site

Yi Xin Huo; Bei Yan Nan; Cong Hui You; Zhe Xian Tian; Annie Kolb; Yi Ping Wang

doi:10.1111/j.1574-6968.2006.00150.x

FIS activates glnAp2 in Escherichia coli: Role of a DNA bend centered at -55, upstream of the transcription start site

Yi Xin Huo
, Bei Yan Nan
, Cong Hui You
, Zhe Xian Tian
, Annie Kolb
, Yi Ping Wang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a σ⁵⁴-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at -55 with respect to the transcription start site (+1). Binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro. Furthermore, we substituted the FIS-mediated DNA bending with other protein (cAMP receptor protein or integration host factor)-mediated DNA bending, without changing the position of the bending center. In vitro transcription assays indicated that all DNA bends centered at -55 activate transcriptional initiation of glnAp2, especially when linear templates were used.

Original language	English
Pages (from-to)	99-105
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	257
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.2006.00150.x
Publication status	Published - Apr 2006
Externally published	Yes

Keywords

Factor for inversion stimulation (FIS)
Integration host factor (IHF)
Protein-mediated DNA bending
cAMP receptor protein (CRP)
σ-RNA polymerase (Eσ)

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10.1111/j.1574-6968.2006.00150.x

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title = "FIS activates glnAp2 in Escherichia coli: Role of a DNA bend centered at -55, upstream of the transcription start site",

abstract = "A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a σ54-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at -55 with respect to the transcription start site (+1). Binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro. Furthermore, we substituted the FIS-mediated DNA bending with other protein (cAMP receptor protein or integration host factor)-mediated DNA bending, without changing the position of the bending center. In vitro transcription assays indicated that all DNA bends centered at -55 activate transcriptional initiation of glnAp2, especially when linear templates were used.",

keywords = "Factor for inversion stimulation (FIS), Integration host factor (IHF), Protein-mediated DNA bending, cAMP receptor protein (CRP), σ-RNA polymerase (Eσ)",

author = "Huo, \{Yi Xin\} and Nan, \{Bei Yan\} and You, \{Cong Hui\} and Tian, \{Zhe Xian\} and Annie Kolb and Wang, \{Yi Ping\}",

year = "2006",

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doi = "10.1111/j.1574-6968.2006.00150.x",

language = "English",

volume = "257",

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journal = "FEMS Microbiology Letters",

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T1 - FIS activates glnAp2 in Escherichia coli

T2 - Role of a DNA bend centered at -55, upstream of the transcription start site

AU - Huo, Yi Xin

AU - Nan, Bei Yan

AU - You, Cong Hui

AU - Tian, Zhe Xian

AU - Kolb, Annie

AU - Wang, Yi Ping

PY - 2006/4

Y1 - 2006/4

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AB - A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a σ54-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at -55 with respect to the transcription start site (+1). Binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro. Furthermore, we substituted the FIS-mediated DNA bending with other protein (cAMP receptor protein or integration host factor)-mediated DNA bending, without changing the position of the bending center. In vitro transcription assays indicated that all DNA bends centered at -55 activate transcriptional initiation of glnAp2, especially when linear templates were used.

KW - Factor for inversion stimulation (FIS)

KW - Integration host factor (IHF)

KW - Protein-mediated DNA bending

KW - cAMP receptor protein (CRP)

KW - σ-RNA polymerase (Eσ)

UR - https://www.scopus.com/pages/publications/33645002427

U2 - 10.1111/j.1574-6968.2006.00150.x

DO - 10.1111/j.1574-6968.2006.00150.x

M3 - Article

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AN - SCOPUS:33645002427

SN - 0378-1097

VL - 257

SP - 99

EP - 105

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

FIS activates glnAp2 in Escherichia coli: Role of a DNA bend centered at -55, upstream of the transcription start site

Abstract

Keywords

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