Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein

Mingshu Zhang; Zhifei Fu; Changqing Li; Anyuan Liu; Dingming Peng; Fudong Xue; Wenting He; Shan Gao; Fan Xu; Dan Xu; Ling Yuan; Fa Zhang; Zhiheng Xu; Tao Xu; Pingyong Xu

doi:10.1021/acs.nanolett.9b02855

Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein

Mingshu Zhang, Zhifei Fu, Changqing Li, Anyuan Liu, Dingming Peng, Fudong Xue, Wenting He, Shan Gao, Fan Xu, Dan Xu, Ling Yuan, Fa Zhang, Zhiheng Xu, Tao Xu^*, Pingyong Xu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1-0.25 s) with large field-of-view (76 × 9.4 μm²-76 × 31.4 μm²) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific "parallel three-pillar" structure in the neuronal-glial cell junction, helping to elucidate glial cell "locking" and support of neurons during Drosophila embryogenesis.

Original language	English
Pages (from-to)	2197-2208
Number of pages	12
Journal	Nano Letters
Volume	20
Issue number	4
DOIs	https://doi.org/10.1021/acs.nanolett.9b02855
Publication status	Published - 8 Apr 2020
Externally published	Yes

Keywords

Drosophila embryos
Photoconvertible fluorescent protein
Quick-SIMBA
live super-resolution microscopy
pcStar

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10.1021/acs.nanolett.9b02855

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title = "Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein",

abstract = "Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1-0.25 s) with large field-of-view (76 × 9.4 μm2-76 × 31.4 μm2) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific {"}parallel three-pillar{"} structure in the neuronal-glial cell junction, helping to elucidate glial cell {"}locking{"} and support of neurons during Drosophila embryogenesis.",

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doi = "10.1021/acs.nanolett.9b02855",

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T1 - Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein

AU - Zhang, Mingshu

AU - Fu, Zhifei

AU - Li, Changqing

AU - Liu, Anyuan

AU - Peng, Dingming

AU - Xue, Fudong

AU - He, Wenting

AU - Gao, Shan

AU - Xu, Fan

AU - Xu, Dan

AU - Yuan, Ling

AU - Zhang, Fa

AU - Xu, Zhiheng

AU - Xu, Tao

AU - Xu, Pingyong

PY - 2020/4/8

Y1 - 2020/4/8

N2 - Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1-0.25 s) with large field-of-view (76 × 9.4 μm2-76 × 31.4 μm2) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific "parallel three-pillar" structure in the neuronal-glial cell junction, helping to elucidate glial cell "locking" and support of neurons during Drosophila embryogenesis.

AB - Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1-0.25 s) with large field-of-view (76 × 9.4 μm2-76 × 31.4 μm2) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific "parallel three-pillar" structure in the neuronal-glial cell junction, helping to elucidate glial cell "locking" and support of neurons during Drosophila embryogenesis.

KW - Drosophila embryos

KW - Photoconvertible fluorescent protein

KW - Quick-SIMBA

KW - live super-resolution microscopy

KW - pcStar

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DO - 10.1021/acs.nanolett.9b02855

M3 - Article

C2 - 31576756

AN - SCOPUS:85073154098

SN - 1530-6984

VL - 20

SP - 2197

EP - 2208

JO - Nano Letters

JF - Nano Letters

IS - 4

ER -

Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein

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Keywords

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