Establishment of peptide biomarker screening model in individual aging in vitro by HPLC/ESI-TOF MS

Mei Zhang; Ke Ming Shan; Yu Lin Deng

doi:10.15918/j.tbit1001-0645.2015.supplement1.025

Establishment of peptide biomarker screening model in individual aging in vitro by HPLC/ESI-TOF MS

Mei Zhang
, Ke Ming Shan
, Yu Lin Deng

School of Medical Science and Engineering

Chinese Center for Disease Control and Prevention
State Key Laboratory for Infectious Disease Prevention and Control
Zhejiang University
Norinco Group

Research output: Contribution to journal › Article › peer-review

Abstract

An in vitro aging model was set up by methylglyoxal and human serum albumin based on maillard reaction according to cross-linking theory to evaluate aging degree individually. HPLC-ESI TOF MS and ¹⁸O-labeling technique were employed to quantify modification degree of peptides cleaved from HSA, which can indicate aging degree. The in vitro aging model was set up successfully, and all experimental conditions were optimized. This method was considered to be a good foundation for screening aging biomarkers in the future by quality evaluation of the optimized conditions.

Original language	English
Pages (from-to)	116-118 and 127
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	35
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.025
Publication status	Published - 1 Jul 2015

Keywords

Aging
Biomarker
HPLC-ESI TOF MS
Human serum albumin
O-labeling

Access to Document

10.15918/j.tbit1001-0645.2015.supplement1.025

Cite this

@article{60a02d613d2a4ca6933b6d8e87d6f076,

title = "Establishment of peptide biomarker screening model in individual aging in vitro by HPLC/ESI-TOF MS",

abstract = "An in vitro aging model was set up by methylglyoxal and human serum albumin based on maillard reaction according to cross-linking theory to evaluate aging degree individually. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify modification degree of peptides cleaved from HSA, which can indicate aging degree. The in vitro aging model was set up successfully, and all experimental conditions were optimized. This method was considered to be a good foundation for screening aging biomarkers in the future by quality evaluation of the optimized conditions.",

keywords = "Aging, Biomarker, HPLC-ESI TOF MS, Human serum albumin, O-labeling",

author = "Mei Zhang and Shan, \{Ke Ming\} and Deng, \{Yu Lin\}",

year = "2015",

month = jul,

day = "1",

doi = "10.15918/j.tbit1001-0645.2015.supplement1.025",

language = "English",

volume = "35",

pages = "116--118 and 127",

journal = "Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology",

issn = "1001-0645",

publisher = "Beijing Institute of Technology",

}

TY - JOUR

T1 - Establishment of peptide biomarker screening model in individual aging in vitro by HPLC/ESI-TOF MS

AU - Zhang, Mei

AU - Shan, Ke Ming

AU - Deng, Yu Lin

PY - 2015/7/1

Y1 - 2015/7/1

N2 - An in vitro aging model was set up by methylglyoxal and human serum albumin based on maillard reaction according to cross-linking theory to evaluate aging degree individually. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify modification degree of peptides cleaved from HSA, which can indicate aging degree. The in vitro aging model was set up successfully, and all experimental conditions were optimized. This method was considered to be a good foundation for screening aging biomarkers in the future by quality evaluation of the optimized conditions.

AB - An in vitro aging model was set up by methylglyoxal and human serum albumin based on maillard reaction according to cross-linking theory to evaluate aging degree individually. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify modification degree of peptides cleaved from HSA, which can indicate aging degree. The in vitro aging model was set up successfully, and all experimental conditions were optimized. This method was considered to be a good foundation for screening aging biomarkers in the future by quality evaluation of the optimized conditions.

KW - Aging

KW - Biomarker

KW - HPLC-ESI TOF MS

KW - Human serum albumin

KW - O-labeling

UR - https://www.scopus.com/pages/publications/84943379545

U2 - 10.15918/j.tbit1001-0645.2015.supplement1.025

DO - 10.15918/j.tbit1001-0645.2015.supplement1.025

M3 - Article

AN - SCOPUS:84943379545

SN - 1001-0645

VL - 35

SP - 116-118 and 127

JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Establishment of peptide biomarker screening model in individual aging in vitro by HPLC/ESI-TOF MS

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this