Engineering a self-navigated MnARK nanovaccine for inducing potent protective immunity against novel coronavirus

Yaling Wang; Yuping Xie; Jia Luo; Mengyu Guo; Xuhao Hu; Xi Chen; Ziwei Chen; Xinyi Lu; Lichun Mao; Kai Zhang; Liangnian Wei; Yunfei Ma; Ruixin Wang; Jia Zhou; Chunyan He; Yufang Zhang; Ye Zhang; Sisi Chen; Lijuan Shen; Yun Chen; Nasha Qiu; Ying Liu; Yanyan Cui; Guoyang Liao; Ye Liu; Chunying Chen

doi:10.1016/j.nantod.2021.101139

Engineering a self-navigated MnARK nanovaccine for inducing potent protective immunity against novel coronavirus

Yaling Wang
, Yuping Xie
, Jia Luo
, Mengyu Guo
, Xuhao Hu
, Xi Chen
, Ziwei Chen
, Xinyi Lu
, Lichun Mao
, Kai Zhang
, Liangnian Wei
, Yunfei Ma
, Ruixin Wang
, Jia Zhou
, Chunyan He
, Yufang Zhang
, Ye Zhang
, Sisi Chen
, Lijuan Shen
, Yun Chen

Nasha Qiu, Ying Liu, Yanyan Cui, Guoyang Liao, Ye Liu^*, Chunying Chen^*

^*Corresponding author for this work

National Center for Nanoscience and Technology
GBA Research Innovation Institute for Nanotechnology
Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College
University of Chinese Academy of Sciences
Zhengzhou University
CAS - Institute of High Energy Physics
Nanjing Medical University
Kunming Medical College
Beijing Institute of Technology
Chinese Academy of Medical Sciences

Research output: Contribution to journal › Article › peer-review

108 Citations (Scopus)

Abstract

Effective vaccines are vital to fight against the COVID-19 global pandemic. As a critical component of a subunit vaccine, the adjuvant is responsible for strengthening the antigen-induced immune responses. Here, we present a new nanovaccine that comprising the Receptor-Binding Domain (RBD) of spike protein and the manganese nanoadjuvant (MnARK), which induces humoral and cellular responses. Notably, even at a 5-fold lower antigen dose and with fewer injections, the MnARK vaccine immunized mice showed stronger neutralizing abilities against the infection of the pseudovirus (~270-fold) and live coronavirus (>8-fold) in vitro than that of Alum-adsorbed RBD vaccine (Alu-RBD). Furthermore, we found that the effective co-delivery of RBD antigen and MnARK to lymph nodes (LNs) elicited an increased cellular internalization and the activation of immune cells, including DCs, CD4⁺ and CD8⁺ T lymphocytes. Our findings highlight the importance of MnARK adjuvant in the design of novel coronavirus vaccines and provide a rationale strategy to design protective vaccines through promoting cellular internalization and the activation of immune-related pathways.

Original language	English
Article number	101139
Journal	Nano Today
Volume	38
DOIs	https://doi.org/10.1016/j.nantod.2021.101139
Publication status	Published - Jun 2021
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/j.nantod.2021.101139

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Wang, Y., Xie, Y., Luo, J., Guo, M., Hu, X., Chen, X., Chen, Z., Lu, X., Mao, L., Zhang, K., Wei, L., Ma, Y., Wang, R., Zhou, J., He, C., Zhang, Y., Zhang, Y., Chen, S., Shen, L., ... Chen, C. (2021). Engineering a self-navigated MnARK nanovaccine for inducing potent protective immunity against novel coronavirus. Nano Today, 38, Article 101139. https://doi.org/10.1016/j.nantod.2021.101139

@article{c5750b8b5ad2458f96b369b3e72d961e,

title = "Engineering a self-navigated MnARK nanovaccine for inducing potent protective immunity against novel coronavirus",

abstract = "Effective vaccines are vital to fight against the COVID-19 global pandemic. As a critical component of a subunit vaccine, the adjuvant is responsible for strengthening the antigen-induced immune responses. Here, we present a new nanovaccine that comprising the Receptor-Binding Domain (RBD) of spike protein and the manganese nanoadjuvant (MnARK), which induces humoral and cellular responses. Notably, even at a 5-fold lower antigen dose and with fewer injections, the MnARK vaccine immunized mice showed stronger neutralizing abilities against the infection of the pseudovirus (\textasciitilde{}270-fold) and live coronavirus (>8-fold) in vitro than that of Alum-adsorbed RBD vaccine (Alu-RBD). Furthermore, we found that the effective co-delivery of RBD antigen and MnARK to lymph nodes (LNs) elicited an increased cellular internalization and the activation of immune cells, including DCs, CD4+ and CD8+ T lymphocytes. Our findings highlight the importance of MnARK adjuvant in the design of novel coronavirus vaccines and provide a rationale strategy to design protective vaccines through promoting cellular internalization and the activation of immune-related pathways.",

author = "Yaling Wang and Yuping Xie and Jia Luo and Mengyu Guo and Xuhao Hu and Xi Chen and Ziwei Chen and Xinyi Lu and Lichun Mao and Kai Zhang and Liangnian Wei and Yunfei Ma and Ruixin Wang and Jia Zhou and Chunyan He and Yufang Zhang and Ye Zhang and Sisi Chen and Lijuan Shen and Yun Chen and Nasha Qiu and Ying Liu and Yanyan Cui and Guoyang Liao and Ye Liu and Chunying Chen",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",

year = "2021",

month = jun,

doi = "10.1016/j.nantod.2021.101139",

language = "English",

volume = "38",

journal = "Nano Today",

issn = "1748-0132",

publisher = "Elsevier B.V.",

}

Wang, Y, Xie, Y, Luo, J, Guo, M, Hu, X, Chen, X, Chen, Z, Lu, X, Mao, L, Zhang, K, Wei, L, Ma, Y, Wang, R, Zhou, J, He, C, Zhang, Y, Zhang, Y, Chen, S, Shen, L, Chen, Y, Qiu, N, Liu, Y, Cui, Y, Liao, G, Liu, Y & Chen, C 2021, 'Engineering a self-navigated MnARK nanovaccine for inducing potent protective immunity against novel coronavirus', Nano Today, vol. 38, 101139. https://doi.org/10.1016/j.nantod.2021.101139

TY - JOUR

T1 - Engineering a self-navigated MnARK nanovaccine for inducing potent protective immunity against novel coronavirus

AU - Wang, Yaling

AU - Xie, Yuping

AU - Luo, Jia

AU - Guo, Mengyu

AU - Hu, Xuhao

AU - Chen, Xi

AU - Chen, Ziwei

AU - Lu, Xinyi

AU - Mao, Lichun

AU - Zhang, Kai

AU - Wei, Liangnian

AU - Ma, Yunfei

AU - Wang, Ruixin

AU - Zhou, Jia

AU - He, Chunyan

AU - Zhang, Yufang

AU - Zhang, Ye

AU - Chen, Sisi

AU - Shen, Lijuan

AU - Chen, Yun

AU - Qiu, Nasha

AU - Liu, Ying

AU - Cui, Yanyan

AU - Liao, Guoyang

AU - Liu, Ye

AU - Chen, Chunying

PY - 2021/6

Y1 - 2021/6

N2 - Effective vaccines are vital to fight against the COVID-19 global pandemic. As a critical component of a subunit vaccine, the adjuvant is responsible for strengthening the antigen-induced immune responses. Here, we present a new nanovaccine that comprising the Receptor-Binding Domain (RBD) of spike protein and the manganese nanoadjuvant (MnARK), which induces humoral and cellular responses. Notably, even at a 5-fold lower antigen dose and with fewer injections, the MnARK vaccine immunized mice showed stronger neutralizing abilities against the infection of the pseudovirus (~270-fold) and live coronavirus (>8-fold) in vitro than that of Alum-adsorbed RBD vaccine (Alu-RBD). Furthermore, we found that the effective co-delivery of RBD antigen and MnARK to lymph nodes (LNs) elicited an increased cellular internalization and the activation of immune cells, including DCs, CD4+ and CD8+ T lymphocytes. Our findings highlight the importance of MnARK adjuvant in the design of novel coronavirus vaccines and provide a rationale strategy to design protective vaccines through promoting cellular internalization and the activation of immune-related pathways.

AB - Effective vaccines are vital to fight against the COVID-19 global pandemic. As a critical component of a subunit vaccine, the adjuvant is responsible for strengthening the antigen-induced immune responses. Here, we present a new nanovaccine that comprising the Receptor-Binding Domain (RBD) of spike protein and the manganese nanoadjuvant (MnARK), which induces humoral and cellular responses. Notably, even at a 5-fold lower antigen dose and with fewer injections, the MnARK vaccine immunized mice showed stronger neutralizing abilities against the infection of the pseudovirus (~270-fold) and live coronavirus (>8-fold) in vitro than that of Alum-adsorbed RBD vaccine (Alu-RBD). Furthermore, we found that the effective co-delivery of RBD antigen and MnARK to lymph nodes (LNs) elicited an increased cellular internalization and the activation of immune cells, including DCs, CD4+ and CD8+ T lymphocytes. Our findings highlight the importance of MnARK adjuvant in the design of novel coronavirus vaccines and provide a rationale strategy to design protective vaccines through promoting cellular internalization and the activation of immune-related pathways.

UR - https://www.scopus.com/pages/publications/85103091495

U2 - 10.1016/j.nantod.2021.101139

DO - 10.1016/j.nantod.2021.101139

M3 - Article

AN - SCOPUS:85103091495

SN - 1748-0132

VL - 38

JO - Nano Today

JF - Nano Today

M1 - 101139

ER -

Engineering a self-navigated MnARK nanovaccine for inducing potent protective immunity against novel coronavirus

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