Electroporation system based on MEMS micro-electrode chip

Hao Yan; Zewen Wei; Xueming Li; Deyao Zhao; Zicai Liang; Zhihong Li

doi:10.3969/j.issn.1004-1699.2012.03.007

Electroporation system based on MEMS micro-electrode chip

Hao Yan^*, Zewen Wei, Xueming Li, Deyao Zhao, Zicai Liang, Zhihong Li

^*Corresponding author for this work

Peking University

Research output: Contribution to journal › Article › peer-review

Abstract

We demanstrated an cell electroporation system based on Au electrode microfabricated chip. We realized high efficiency electroporation in both standard expression cell(HEK-293A)and hard-to-transfected cell(3T3-L1), with a transfection rate of 90% and 80%, respectively. The operation system is friendly, With manual mode and auto mode. The unique electrode design promises much low applied voltage, low cost and easy operation.

Original language	English
Pages (from-to)	319-321
Number of pages	3
Journal	Chinese Journal of Sensors and Actuators
Volume	25
Issue number	3
DOIs	https://doi.org/10.3969/j.issn.1004-1699.2012.03.007
Publication status	Published - Mar 2012
Externally published	Yes

Keywords

Bio-MEMS
Cell electroporation
Electroporation system
Low voltage
Micro fabrication

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10.3969/j.issn.1004-1699.2012.03.007

Cite this

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title = "Electroporation system based on MEMS micro-electrode chip",

abstract = "We demanstrated an cell electroporation system based on Au electrode microfabricated chip. We realized high efficiency electroporation in both standard expression cell(HEK-293A)and hard-to-transfected cell(3T3-L1), with a transfection rate of 90\% and 80\%, respectively. The operation system is friendly, With manual mode and auto mode. The unique electrode design promises much low applied voltage, low cost and easy operation.",

keywords = "Bio-MEMS, Cell electroporation, Electroporation system, Low voltage, Micro fabrication",

author = "Hao Yan and Zewen Wei and Xueming Li and Deyao Zhao and Zicai Liang and Zhihong Li",

year = "2012",

month = mar,

doi = "10.3969/j.issn.1004-1699.2012.03.007",

language = "English",

volume = "25",

pages = "319--321",

journal = "Chinese Journal of Sensors and Actuators",

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TY - JOUR

T1 - Electroporation system based on MEMS micro-electrode chip

AU - Yan, Hao

AU - Wei, Zewen

AU - Li, Xueming

AU - Zhao, Deyao

AU - Liang, Zicai

AU - Li, Zhihong

PY - 2012/3

Y1 - 2012/3

N2 - We demanstrated an cell electroporation system based on Au electrode microfabricated chip. We realized high efficiency electroporation in both standard expression cell(HEK-293A)and hard-to-transfected cell(3T3-L1), with a transfection rate of 90% and 80%, respectively. The operation system is friendly, With manual mode and auto mode. The unique electrode design promises much low applied voltage, low cost and easy operation.

AB - We demanstrated an cell electroporation system based on Au electrode microfabricated chip. We realized high efficiency electroporation in both standard expression cell(HEK-293A)and hard-to-transfected cell(3T3-L1), with a transfection rate of 90% and 80%, respectively. The operation system is friendly, With manual mode and auto mode. The unique electrode design promises much low applied voltage, low cost and easy operation.

KW - Bio-MEMS

KW - Cell electroporation

KW - Electroporation system

KW - Low voltage

KW - Micro fabrication

UR - https://www.scopus.com/pages/publications/84861921447

U2 - 10.3969/j.issn.1004-1699.2012.03.007

DO - 10.3969/j.issn.1004-1699.2012.03.007

M3 - Article

AN - SCOPUS:84861921447

SN - 1004-1699

VL - 25

SP - 319

EP - 321

JO - Chinese Journal of Sensors and Actuators

JF - Chinese Journal of Sensors and Actuators

IS - 3

ER -

Electroporation system based on MEMS micro-electrode chip

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Keywords

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