Electrodeposition of cell-laden alginate-PLL hydrogel structures for spatially selective entrapment

Zeyang Liu, Masaru Takeuchi, Masahiro Nakajima, Toshio Fukuda, Yasuhisa Hasegawa, Qiang Huang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Citations (Scopus)

Abstract

In this study, cell-laden alginate-poly-L-lysine (PLL) hydrogel structures with arbitrary shapes were constructed based on electrodeposition method. Electrolysis of water in alginate solutions with calcium carbonate particles induced alginate gelation on micro-patterned anode electrode, and cell-laden alginate gel structures were formed. The several different shapes of gel were generated at one time by predefined micro-patterns. The micro-patterned electrode was fabricated by coating photoresist on Fluorine-doped tin oxide (FTO) glass slide. Alginate-PLL encapsulation technique was introduced to this platform forming alginate-PLL hydrogel structures. This proposed method can lead to more accurate and creative studies of fabricating cell-laden scaffold for tissue engineering.

Original languageEnglish
Title of host publicationIROS Hamburg 2015 - Conference Digest
Subtitle of host publicationIEEE/RSJ International Conference on Intelligent Robots and Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1379-1384
Number of pages6
ISBN (Electronic)9781479999941
DOIs
Publication statusPublished - 11 Dec 2015
EventIEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2015 - Hamburg, Germany
Duration: 28 Sept 20152 Oct 2015

Publication series

NameIEEE International Conference on Intelligent Robots and Systems
Volume2015-December
ISSN (Print)2153-0858
ISSN (Electronic)2153-0866

Conference

ConferenceIEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2015
Country/TerritoryGermany
CityHamburg
Period28/09/152/10/15

Keywords

  • Calcium
  • Cells (biology)
  • Electrodes
  • Fabrication
  • Fluorescence
  • Phase locked loops
  • Shape

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