Efficient biosynthesis of glycyrrhetic acid 3-O-mono-β-d-glucuronide (GAMG) in water-miscible ionic liquid by immobilized whole cells of Penicillium purpurogenum Li-3 in alginate gel

Five immobilizing materials were investigated for the catalytic efficiency of whole cells of Penicillium purpurogenum Li-3 (w-PGUS) and alginate gel was found the best polymer for the biotransformation of glycyrrhizin (GL) into glycyrrhetic acid 3-O-mono-β-d-glucuronide (GAMG). The activity of compositionally optimized w-PGUS alginate beads was examined in different ionic liquids (ILs). The 30% (v/v) hydrophilic IL [BMIM]BF₄ in reaction mixture exhibited an explicit biocompatibility with alginate support; consequently aggrandized the catalytic efficiency of the immobilized w-PGUS compared with pure buffer and ILs media. The optimal biotransformation conditions of the immobilized w-PGUS were evaluated and compared with buffer and IL co-solvent media: including w-PGUS beads concentration (70g/l vs. 80g/l), substrate concentration (5mM vs. 4mM), temperature (45°C vs. 45°C), pH (5.4 vs. 5.6), and shaking speed (180rpm vs. 160rpm). Under the optimized conditions; the yield of GAMG was 2.62g/l after 62h in IL co-solvent medium compared to 2.34g/l after 72h in buffer medium. The immobilized w-PGUS also retained 45.80% and 18.13% of their original activity in IL co-solvent and buffer media respectively; after ten repeated batches displaying high operational stability for GL biotransformation.