Effects of stem cell therapy on protein profile of parkinsonian rats using an18O-labeling quantitative proteomic approach

Yahui Liu; Kefu Liu; Wei Qin; Chenghao Liu; Xiaowei Zheng; Yulin Deng; Hong Qing

doi:10.1002/pmic.201500421

Effects of stem cell therapy on protein profile of parkinsonian rats using an¹⁸O-labeling quantitative proteomic approach

Yahui Liu, Kefu Liu, Wei Qin, Chenghao Liu, Xiaowei Zheng, Yulin Deng, Hong Qing^*

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The application of neural stem cell (NSC) research to neurodegenerative diseases has led to promising clinical trials. Currently, NSC therapy is most promising for Parkinson's disease (PD). We conducted behavioral tests and immunoassays for the profiling of a PD model in rats to assess the therapeutic effects of NSC treatments. Further, using a multiple sample comparison workflow, combined with ¹⁸O-labeled proteome mixtures, we compared the differentially expressed proteins from control, PD, and NSC-treated PD rats. The results were analyzed bioinformatically and verified by Western blot. Based on our initial findings, we believe that the proteomic approach is a valuable tool in evaluating the therapeutic effects of NSC transplantation on neurodegenerative disorders.

Original language	English
Pages (from-to)	1023-1032
Number of pages	10
Journal	Proteomics
Volume	16
Issue number	6
DOIs	https://doi.org/10.1002/pmic.201500421
Publication status	Published - 1 Mar 2016

Keywords

Animal proteomics
Neural stem cell
O-labeling
Parkinson's disease
Quantitative proteomics
Therapy

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10.1002/pmic.201500421

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abstract = "The application of neural stem cell (NSC) research to neurodegenerative diseases has led to promising clinical trials. Currently, NSC therapy is most promising for Parkinson's disease (PD). We conducted behavioral tests and immunoassays for the profiling of a PD model in rats to assess the therapeutic effects of NSC treatments. Further, using a multiple sample comparison workflow, combined with 18O-labeled proteome mixtures, we compared the differentially expressed proteins from control, PD, and NSC-treated PD rats. The results were analyzed bioinformatically and verified by Western blot. Based on our initial findings, we believe that the proteomic approach is a valuable tool in evaluating the therapeutic effects of NSC transplantation on neurodegenerative disorders.",

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AU - Liu, Yahui

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AU - Qin, Wei

AU - Liu, Chenghao

AU - Zheng, Xiaowei

AU - Deng, Yulin

AU - Qing, Hong

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AB - The application of neural stem cell (NSC) research to neurodegenerative diseases has led to promising clinical trials. Currently, NSC therapy is most promising for Parkinson's disease (PD). We conducted behavioral tests and immunoassays for the profiling of a PD model in rats to assess the therapeutic effects of NSC treatments. Further, using a multiple sample comparison workflow, combined with 18O-labeled proteome mixtures, we compared the differentially expressed proteins from control, PD, and NSC-treated PD rats. The results were analyzed bioinformatically and verified by Western blot. Based on our initial findings, we believe that the proteomic approach is a valuable tool in evaluating the therapeutic effects of NSC transplantation on neurodegenerative disorders.

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KW - Neural stem cell

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Effects of stem cell therapy on protein profile of parkinsonian rats using an¹⁸O-labeling quantitative proteomic approach

Abstract

Keywords

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