TY - JOUR
T1 - Effects of Mannitol on the Growth, Metabolism, and Butenyl-Spinosyn Biosynthesis of Saccharopolyspora pogona
AU - Guo, Chao
AU - Li, Xinying
AU - Chen, Xia
AU - Su, Chang
AU - Li, Chun
AU - Feng, Yongjun
AU - Wang, Chao
N1 - Publisher Copyright:
© 2025 American Chemical Society.
PY - 2025
Y1 - 2025
N2 - The metabolic pathways of high-yielding strains developed through physical and chemical mutagenesis can be modified, particularly those involved in carbon metabolism. The distribution of nutrients in the fermentation medium is likely to be a key bottleneck in further improving yields. In this study, we screened different carbon sources for the high-yield Saccharopolyspora pogona ASAGF19 strain and investigated the underlying mechanisms. The results demonstrated that mannitol was the optimal carbon source, increasing biomass and butenyl-spinosyn production by 1.58- and 1.88-fold, respectively, compared to glucose. The specific growth rate and carbon source utilization efficiency in the mannitol group were 7.37- and 1.68-fold higher than in the glucose group at 96 h of fermentation. Additionally, at 72 h, the synthesis rate of butenyl-spinosyn and the carbon yield in the mannitol group increased by 2.02- and 1.89-fold, respectively. Mannitol also enhanced the transcription of genes involved in the biosynthesis of butenyl-spinosyn, elevated NADPH levels during the early stage of fermentation, and maintained cellular redox homeostasis. Its supplementation in the fermentation medium also promoted butenyl-spinosyn biosynthesis. The increased nicotinamide adenine dinucleotide phosphate supply in the early stage, along with maintained intracellular redox balance, likely contributed to the highly efficient biosynthesis of butenyl-spinosyn in S. pogona ASAGF19. This study is the first to investigate the mechanism by which mannitol enhances butenyl-spinosyn production.
AB - The metabolic pathways of high-yielding strains developed through physical and chemical mutagenesis can be modified, particularly those involved in carbon metabolism. The distribution of nutrients in the fermentation medium is likely to be a key bottleneck in further improving yields. In this study, we screened different carbon sources for the high-yield Saccharopolyspora pogona ASAGF19 strain and investigated the underlying mechanisms. The results demonstrated that mannitol was the optimal carbon source, increasing biomass and butenyl-spinosyn production by 1.58- and 1.88-fold, respectively, compared to glucose. The specific growth rate and carbon source utilization efficiency in the mannitol group were 7.37- and 1.68-fold higher than in the glucose group at 96 h of fermentation. Additionally, at 72 h, the synthesis rate of butenyl-spinosyn and the carbon yield in the mannitol group increased by 2.02- and 1.89-fold, respectively. Mannitol also enhanced the transcription of genes involved in the biosynthesis of butenyl-spinosyn, elevated NADPH levels during the early stage of fermentation, and maintained cellular redox homeostasis. Its supplementation in the fermentation medium also promoted butenyl-spinosyn biosynthesis. The increased nicotinamide adenine dinucleotide phosphate supply in the early stage, along with maintained intracellular redox balance, likely contributed to the highly efficient biosynthesis of butenyl-spinosyn in S. pogona ASAGF19. This study is the first to investigate the mechanism by which mannitol enhances butenyl-spinosyn production.
KW - butenyl-spinosyn
KW - carbon source
KW - mannitol
KW - medium optimization
KW - nicotinamide adenine dinucleotide phosphate
KW - Saccharopolyspora pogona
UR - http://www.scopus.com/inward/record.url?scp=105001675918&partnerID=8YFLogxK
U2 - 10.1021/acsagscitech.5c00011
DO - 10.1021/acsagscitech.5c00011
M3 - Article
AN - SCOPUS:105001675918
SN - 2692-1952
JO - ACS Agricultural Science and Technology
JF - ACS Agricultural Science and Technology
ER -