TY - JOUR
T1 - Early screening and staging of melanoma using blood based on laser-induced breakdown spectroscopy
AU - Zhao, Zhifang
AU - Xu, Xiangjun
AU - Bao, Mengyu
AU - Zheng, Yongyue
AU - Luo, Tianzhong
AU - Lu, Bingheng
AU - Teng, Geer
AU - Wang, Qianqian
AU - Nouman Khan, Muhammad
AU - Yong, Jun
N1 - Publisher Copyright:
© 2024
PY - 2024/8
Y1 - 2024/8
N2 - For melanoma, early screening could increase the cure rate, while staging helps to make treatment strategies. Blood sampling has advantages of little damage, convenient operation and low cost, which combined with laser-induced breakdown spectroscopy (LIBS) has been utilized for tumor diagnoses. Here, we proposed to accurately attain early screening and staging of melanoma blood using LIBS. The serum was collected from 25 melanoma mice and 10 healthy controls on the 7th, 14th, 21st and 28th days. Compared with k nearest neighbor (kNN), support vector machine (SVM) and back propagation neural network (BPNN) models, the adaptive boosting of BPNN (BP_AdaBoost) models had the best accuracies of 83.37 % for early screening and 96.18 % for staging, respectively. Using mutual information (MI) method to select features, the accuracies of BP_AdaBoost models were improved to 86.11 % for early screening and 96.91 % for staging, respectively. Besides, the difference significance of elements and molecular bands in the serum was examined by the Kruskal-Wallis (K-W) test. The test results showed that obvious differences of Ca and Na existed in both early screening and staging, while K and Mg made significant differences in staging, consistent with roles of Ca and Na in the whole process of tumor development and roles of K and Mg in tumor proliferation and metastasis. Overall, all results demonstrated that early screening and staging of melanoma could be accurately realized using blood based on LIBS.
AB - For melanoma, early screening could increase the cure rate, while staging helps to make treatment strategies. Blood sampling has advantages of little damage, convenient operation and low cost, which combined with laser-induced breakdown spectroscopy (LIBS) has been utilized for tumor diagnoses. Here, we proposed to accurately attain early screening and staging of melanoma blood using LIBS. The serum was collected from 25 melanoma mice and 10 healthy controls on the 7th, 14th, 21st and 28th days. Compared with k nearest neighbor (kNN), support vector machine (SVM) and back propagation neural network (BPNN) models, the adaptive boosting of BPNN (BP_AdaBoost) models had the best accuracies of 83.37 % for early screening and 96.18 % for staging, respectively. Using mutual information (MI) method to select features, the accuracies of BP_AdaBoost models were improved to 86.11 % for early screening and 96.91 % for staging, respectively. Besides, the difference significance of elements and molecular bands in the serum was examined by the Kruskal-Wallis (K-W) test. The test results showed that obvious differences of Ca and Na existed in both early screening and staging, while K and Mg made significant differences in staging, consistent with roles of Ca and Na in the whole process of tumor development and roles of K and Mg in tumor proliferation and metastasis. Overall, all results demonstrated that early screening and staging of melanoma could be accurately realized using blood based on LIBS.
KW - Blood
KW - Early screening and staging
KW - LIBS
KW - Melanoma
UR - http://www.scopus.com/inward/record.url?scp=85195651442&partnerID=8YFLogxK
U2 - 10.1016/j.microc.2024.110955
DO - 10.1016/j.microc.2024.110955
M3 - Article
AN - SCOPUS:85195651442
SN - 0026-265X
VL - 203
JO - Microchemical Journal
JF - Microchemical Journal
M1 - 110955
ER -