Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection ofStaphylococcus aureus

Shu Wang; Wanzhu Shen; Shuai Zheng; Zhigang Li; Chongwen Wang; Long Zhang; Yong Liu

doi:10.1039/d1ra01085a

Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection ofStaphylococcus aureus

Shu Wang
, Wanzhu Shen
, Shuai Zheng
, Zhigang Li
, Chongwen Wang^*
, Long Zhang
, Yong Liu

^*Corresponding author for this work

Chinese Academy of Sciences
University of Science and Technology of China
Anhui Agricultural University
Academy of Military Medical Science China

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

This paper reports a colorimetric-fluorescent dual-signal lateral flow assay (LFA) based on vancomycin (Van)-modified SiO₂-Au-QD tags for sensitive and quantitative detection ofStaphylococcus aureus(S. aureus). The combination of high-performance Van-tags and detection antibodies integrated into the LFA system produced assays with high sensitivity and specificity. The visualization limit of the colorimetric signal and the detection limit of the fluorescence signal of the proposed method forS. aureuscan reach 10⁴and 100 cells mL^-1, respectively.

Original language	English
Pages (from-to)	13297-13303
Number of pages	7
Journal	RSC Advances
Volume	11
Issue number	22
DOIs	https://doi.org/10.1039/d1ra01085a
Publication status	Published - 9 Apr 2021
Externally published	Yes

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title = "Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection ofStaphylococcus aureus",

abstract = "This paper reports a colorimetric-fluorescent dual-signal lateral flow assay (LFA) based on vancomycin (Van)-modified SiO2-Au-QD tags for sensitive and quantitative detection ofStaphylococcus aureus(S. aureus). The combination of high-performance Van-tags and detection antibodies integrated into the LFA system produced assays with high sensitivity and specificity. The visualization limit of the colorimetric signal and the detection limit of the fluorescence signal of the proposed method forS. aureuscan reach 104and 100 cells mL-1, respectively.",

author = "Shu Wang and Wanzhu Shen and Shuai Zheng and Zhigang Li and Chongwen Wang and Long Zhang and Yong Liu",

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doi = "10.1039/d1ra01085a",

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T1 - Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection ofStaphylococcus aureus

AU - Wang, Shu

AU - Shen, Wanzhu

AU - Zheng, Shuai

AU - Li, Zhigang

AU - Wang, Chongwen

AU - Zhang, Long

AU - Liu, Yong

PY - 2021/4/9

Y1 - 2021/4/9

N2 - This paper reports a colorimetric-fluorescent dual-signal lateral flow assay (LFA) based on vancomycin (Van)-modified SiO2-Au-QD tags for sensitive and quantitative detection ofStaphylococcus aureus(S. aureus). The combination of high-performance Van-tags and detection antibodies integrated into the LFA system produced assays with high sensitivity and specificity. The visualization limit of the colorimetric signal and the detection limit of the fluorescence signal of the proposed method forS. aureuscan reach 104and 100 cells mL-1, respectively.

AB - This paper reports a colorimetric-fluorescent dual-signal lateral flow assay (LFA) based on vancomycin (Van)-modified SiO2-Au-QD tags for sensitive and quantitative detection ofStaphylococcus aureus(S. aureus). The combination of high-performance Van-tags and detection antibodies integrated into the LFA system produced assays with high sensitivity and specificity. The visualization limit of the colorimetric signal and the detection limit of the fluorescence signal of the proposed method forS. aureuscan reach 104and 100 cells mL-1, respectively.

UR - https://www.scopus.com/pages/publications/85104270378

U2 - 10.1039/d1ra01085a

DO - 10.1039/d1ra01085a

M3 - Article

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SN - 2046-2069

VL - 11

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EP - 13303

JO - RSC Advances

JF - RSC Advances

IS - 22

ER -

Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection ofStaphylococcus aureus

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