Dual-Bubble Coordinated Acoustic Micromanipulator for Multidirectional Object Rotation

Micromanipulation techniques struggle to achieve three-dimensional rotational control at the microscale without compromising biocompatibility or spatial flexibility. Conventional methods based on mechanical contact, optical forces, or confined microfluidics constrain dynamic reconfiguration and surgical accessibility. Here, we introduce a dual-bubble acoustic micromanipulator that enables multidirectional rotation through controlled hydrodynamic fields. By placing oscillating microbubbles at the tips of micropipettes, this system creates adjustable vortex patterns: a single microbubble generates toroidal flows for out-of-plane rotation, while two microbubbles produce shear forces for in-plane spinning. This approach uses simple mechanical adjustments to control rotational axes in open fluid environments, without needing frequency modulation or phase synchronization. Flow-field simulations and experiments with polystyrene microspheres confirm deterministic orientation control, and tests with shrimp embryos demonstrate rotation at clinically relevant speeds. The open architecture integrates seamlessly with standard microscopy and robotic injection systems, offering a non-contact, precise tool for applications such as polar body alignment, intracellular surgery, and 3-D imaging.