Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins

Yalan Zhu; Ang Gao; Qi Zhan; Yong Wang; Han Feng; Songqing Liu; Guangxia Gao; Alexander Serganov; Pu Gao

doi:10.1016/j.molcel.2019.01.038

Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins

Yalan Zhu
, Ang Gao
, Qi Zhan
, Yong Wang
, Han Feng
, Songqing Liu
, Guangxia Gao
, Alexander Serganov^*
, Pu Gao

^*Corresponding author for this work

CAS - Institute of Biophysics
University of Chinese Academy of Sciences
New York University

Research output: Contribution to journal › Article › peer-review

91 Citations (Scopus)

Abstract

Zhu et al. report biochemical and structural data that suggest molecular mechanisms of AcrIIC2- and AcrIIC3-mediated inhibition of Cas9. The two inhibitors employ distinct means to block Cas9 activity that include binding to different regions, targeting distinct steps of catalysis, and inhibiting different scopes of Cas9 orthologs.

Original language	English
Pages (from-to)	296-309.e7
Journal	Molecular Cell
Volume	74
Issue number	2
DOIs	https://doi.org/10.1016/j.molcel.2019.01.038
Publication status	Published - 18 Apr 2019
Externally published	Yes

Keywords

AcrIIC1
AcrIIC2
AcrIIC3
CRISPR
Cas9
anti-CRISPR

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10.1016/j.molcel.2019.01.038

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title = "Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins",

abstract = "Zhu et al. report biochemical and structural data that suggest molecular mechanisms of AcrIIC2- and AcrIIC3-mediated inhibition of Cas9. The two inhibitors employ distinct means to block Cas9 activity that include binding to different regions, targeting distinct steps of catalysis, and inhibiting different scopes of Cas9 orthologs.",

keywords = "AcrIIC1, AcrIIC2, AcrIIC3, CRISPR, Cas9, anti-CRISPR",

author = "Yalan Zhu and Ang Gao and Qi Zhan and Yong Wang and Han Feng and Songqing Liu and Guangxia Gao and Alexander Serganov and Pu Gao",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

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doi = "10.1016/j.molcel.2019.01.038",

language = "English",

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T1 - Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins

AU - Zhu, Yalan

AU - Gao, Ang

AU - Zhan, Qi

AU - Wang, Yong

AU - Feng, Han

AU - Liu, Songqing

AU - Gao, Guangxia

AU - Serganov, Alexander

AU - Gao, Pu

PY - 2019/4/18

Y1 - 2019/4/18

N2 - Zhu et al. report biochemical and structural data that suggest molecular mechanisms of AcrIIC2- and AcrIIC3-mediated inhibition of Cas9. The two inhibitors employ distinct means to block Cas9 activity that include binding to different regions, targeting distinct steps of catalysis, and inhibiting different scopes of Cas9 orthologs.

AB - Zhu et al. report biochemical and structural data that suggest molecular mechanisms of AcrIIC2- and AcrIIC3-mediated inhibition of Cas9. The two inhibitors employ distinct means to block Cas9 activity that include binding to different regions, targeting distinct steps of catalysis, and inhibiting different scopes of Cas9 orthologs.

KW - AcrIIC1

KW - AcrIIC2

KW - AcrIIC3

KW - CRISPR

KW - Cas9

KW - anti-CRISPR

UR - https://www.scopus.com/pages/publications/85064274842

U2 - 10.1016/j.molcel.2019.01.038

DO - 10.1016/j.molcel.2019.01.038

M3 - Article

C2 - 30850331

AN - SCOPUS:85064274842

SN - 1097-2765

VL - 74

SP - 296-309.e7

JO - Molecular Cell

JF - Molecular Cell

IS - 2

ER -

Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins

Abstract

Keywords

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