Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy

Lisheng Lin; Huiyun Lin; Defu Chen; Longchao Chen; Min Wang; Shusen Xie; Ying Gu; Brian C. Wilson; Buhong Li

doi:10.1117/12.2052033

Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy

Lisheng Lin
, Huiyun Lin
, Defu Chen
, Longchao Chen
, Min Wang
, Shusen Xie
, Ying Gu
, Brian C. Wilson
, Buhong Li

Fujian Normal University
General Hospital of People's Liberation Army
Department of Medical Biophysics, University of Toronto, Princess Margaret Cancer Centre

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

17 Citations (Scopus)

Abstract

Singlet oxygen (¹O₂) is commonly recognized to be a major phototoxic component for inducing the biological damage during photodynamic therapy (PDT). In this study, a novel configuration of a thermoelectrically-cooled near-infrared sensitive InGaAs camera was developed for imaging of photodynamically-generated ¹O₂ luminescence. The validation of ¹O₂ luminescence images for solution samples was performed with the model photosensitizer Rose Bengal (RB). Images of ¹O₂ luminescence generated in blood vessels in vivo in a well-controlled dorsal skinfold window chamber model were also recorded during PDT. This study demonstrated the capacity of the newly-developed imaging system for imaging of ¹O₂ luminescence, and the first reported images of ¹O₂ luminescence in blood vessels in vivo. This system has potential for elucidating the mechanisms of vascular targeted PDT.

Original language	English
Title of host publication	Biophotonics
Subtitle of host publication	Photonic Solutions for Better Health Care IV
Publisher	SPIE
ISBN (Print)	9781628410778
DOIs	https://doi.org/10.1117/12.2052033
Publication status	Published - 2014
Externally published	Yes
Event	Biophotonics: Photonic Solutions for Better Health Care IV - Brussels, Belgium Duration: 14 Apr 2014 → 17 Apr 2014

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	9129
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Biophotonics: Photonic Solutions for Better Health Care IV
Country/Territory	Belgium
City	Brussels
Period	14/04/14 → 17/04/14

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10.1117/12.2052033

Cite this

Lin, L., Lin, H., Chen, D., Chen, L., Wang, M., Xie, S., Gu, Y., Wilson, B. C., & Li, B. (2014). Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy. In Biophotonics: Photonic Solutions for Better Health Care IV Article 912920 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9129). SPIE. https://doi.org/10.1117/12.2052033

@inproceedings{f2b739dcfcfe4d958a4146caee2c7060,

title = "Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy",

abstract = "Singlet oxygen (1O2) is commonly recognized to be a major phototoxic component for inducing the biological damage during photodynamic therapy (PDT). In this study, a novel configuration of a thermoelectrically-cooled near-infrared sensitive InGaAs camera was developed for imaging of photodynamically-generated 1O2 luminescence. The validation of 1O2 luminescence images for solution samples was performed with the model photosensitizer Rose Bengal (RB). Images of 1O2 luminescence generated in blood vessels in vivo in a well-controlled dorsal skinfold window chamber model were also recorded during PDT. This study demonstrated the capacity of the newly-developed imaging system for imaging of 1O2 luminescence, and the first reported images of 1O2 luminescence in blood vessels in vivo. This system has potential for elucidating the mechanisms of vascular targeted PDT.",

author = "Lisheng Lin and Huiyun Lin and Defu Chen and Longchao Chen and Min Wang and Shusen Xie and Ying Gu and Wilson, \{Brian C.\} and Buhong Li",

year = "2014",

doi = "10.1117/12.2052033",

language = "English",

isbn = "9781628410778",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE",

booktitle = "Biophotonics",

address = "United States",

note = "Biophotonics: Photonic Solutions for Better Health Care IV ; Conference date: 14-04-2014 Through 17-04-2014",

}

Lin, L, Lin, H, Chen, D, Chen, L, Wang, M, Xie, S, Gu, Y, Wilson, BC & Li, B 2014, Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy. in Biophotonics: Photonic Solutions for Better Health Care IV., 912920, Proceedings of SPIE - The International Society for Optical Engineering, vol. 9129, SPIE, Biophotonics: Photonic Solutions for Better Health Care IV, Brussels, Belgium, 14/04/14. https://doi.org/10.1117/12.2052033

Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy. / Lin, Lisheng; Lin, Huiyun; Chen, Defu et al.
Biophotonics: Photonic Solutions for Better Health Care IV. SPIE, 2014. 912920 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9129).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy

AU - Lin, Lisheng

AU - Lin, Huiyun

AU - Chen, Defu

AU - Chen, Longchao

AU - Wang, Min

AU - Xie, Shusen

AU - Gu, Ying

AU - Wilson, Brian C.

AU - Li, Buhong

PY - 2014

Y1 - 2014

N2 - Singlet oxygen (1O2) is commonly recognized to be a major phototoxic component for inducing the biological damage during photodynamic therapy (PDT). In this study, a novel configuration of a thermoelectrically-cooled near-infrared sensitive InGaAs camera was developed for imaging of photodynamically-generated 1O2 luminescence. The validation of 1O2 luminescence images for solution samples was performed with the model photosensitizer Rose Bengal (RB). Images of 1O2 luminescence generated in blood vessels in vivo in a well-controlled dorsal skinfold window chamber model were also recorded during PDT. This study demonstrated the capacity of the newly-developed imaging system for imaging of 1O2 luminescence, and the first reported images of 1O2 luminescence in blood vessels in vivo. This system has potential for elucidating the mechanisms of vascular targeted PDT.

AB - Singlet oxygen (1O2) is commonly recognized to be a major phototoxic component for inducing the biological damage during photodynamic therapy (PDT). In this study, a novel configuration of a thermoelectrically-cooled near-infrared sensitive InGaAs camera was developed for imaging of photodynamically-generated 1O2 luminescence. The validation of 1O2 luminescence images for solution samples was performed with the model photosensitizer Rose Bengal (RB). Images of 1O2 luminescence generated in blood vessels in vivo in a well-controlled dorsal skinfold window chamber model were also recorded during PDT. This study demonstrated the capacity of the newly-developed imaging system for imaging of 1O2 luminescence, and the first reported images of 1O2 luminescence in blood vessels in vivo. This system has potential for elucidating the mechanisms of vascular targeted PDT.

UR - https://www.scopus.com/pages/publications/84903788605

U2 - 10.1117/12.2052033

DO - 10.1117/12.2052033

M3 - Conference contribution

AN - SCOPUS:84903788605

SN - 9781628410778

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Biophotonics

PB - SPIE

T2 - Biophotonics: Photonic Solutions for Better Health Care IV

Y2 - 14 April 2014 through 17 April 2014

ER -

Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy

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