Direct electrochemistry with nitrate reductase in chitosan films

Yaru Song, Xiaoxia Chen, Hong Wu, Huibo Shao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Stable films made from chitosan (CS) on pyrolytic graphite (PG) electrode gave direct electrochemistry for incorporated enzyme nitrate reductase (NR). Cyclic voltammetry (CV) of NR-CS films showed a pair of well-defined and nearly reversible redox peaks at about -0.430 V vs. SCE at pH 7.0 phosphate buffers, which was considered as the redox of FAD and heme-ion. The electron transfer between NR and PG electrode was greatly facilitated in CS films. Integration of reduction peaks at different scan rates from 0.01 to 0.5 V s-1 gave nearly constant charge values, which is characteristic of thin-larger- electrochemical behavior. The pH of the solution strongly affected the direct electron transfer of NR-CS films. EDTA accelerated the electron transfer, and it was proposed as a stimulant for the system. Reflectance absorption infrared spectra demonstrated that NR retained a nearly native conformation in CS films.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalJournal of the Chinese Chemical Society
Volume51
Issue number1
DOIs
Publication statusPublished - Feb 2004
Externally publishedYes

Keywords

  • Chitosan
  • Cyclic voltammetry
  • Nitrate reductase

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