Development and characterizations of a miniature capillary electrophoresis mass spectrometry system

Muyi He; Zhenhua Xue; Yinna Zhang; Zejian Huang; Xiang Fang; Feng Qu; Zheng Ouyang; Wei Xu

doi:10.1021/ac504868w

Development and characterizations of a miniature capillary electrophoresis mass spectrometry system

Muyi He
, Zhenhua Xue
, Yinna Zhang
, Zejian Huang
, Xiang Fang
, Feng Qu
, Zheng Ouyang
, Wei Xu^*

^*Corresponding author for this work

School of Medical Technology

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

A miniature capillary electrophoresis mass spectrometry (CE/MS) system has been developed in this work. A 100% electrical driven miniaturized CE device was integrated with a miniature MS instrument, which has a discontinuous atmospheric pressure interface (DAPI) for coupling with atmospheric pressure ionization sources. A nanoelectrospray ionization (nano-ESI) source was developed with a sheath liquid interface for coupling the miniature CE and the MS system. A systematic characterization and optimization of the analytical performance have been done. The analysis of isobaric peptides and avoiding charge competition effects in nano-ESI sources have been demonstrated.

Original language	English
Pages (from-to)	2236-2241
Number of pages	6
Journal	Analytical Chemistry
Volume	87
Issue number	4
DOIs	https://doi.org/10.1021/ac504868w
Publication status	Published - 17 Feb 2015

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10.1021/ac504868w

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title = "Development and characterizations of a miniature capillary electrophoresis mass spectrometry system",

abstract = "A miniature capillary electrophoresis mass spectrometry (CE/MS) system has been developed in this work. A 100\% electrical driven miniaturized CE device was integrated with a miniature MS instrument, which has a discontinuous atmospheric pressure interface (DAPI) for coupling with atmospheric pressure ionization sources. A nanoelectrospray ionization (nano-ESI) source was developed with a sheath liquid interface for coupling the miniature CE and the MS system. A systematic characterization and optimization of the analytical performance have been done. The analysis of isobaric peptides and avoiding charge competition effects in nano-ESI sources have been demonstrated.",

author = "Muyi He and Zhenhua Xue and Yinna Zhang and Zejian Huang and Xiang Fang and Feng Qu and Zheng Ouyang and Wei Xu",

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AU - He, Muyi

AU - Xue, Zhenhua

AU - Zhang, Yinna

AU - Huang, Zejian

AU - Fang, Xiang

AU - Qu, Feng

AU - Ouyang, Zheng

AU - Xu, Wei

PY - 2015/2/17

Y1 - 2015/2/17

N2 - A miniature capillary electrophoresis mass spectrometry (CE/MS) system has been developed in this work. A 100% electrical driven miniaturized CE device was integrated with a miniature MS instrument, which has a discontinuous atmospheric pressure interface (DAPI) for coupling with atmospheric pressure ionization sources. A nanoelectrospray ionization (nano-ESI) source was developed with a sheath liquid interface for coupling the miniature CE and the MS system. A systematic characterization and optimization of the analytical performance have been done. The analysis of isobaric peptides and avoiding charge competition effects in nano-ESI sources have been demonstrated.

AB - A miniature capillary electrophoresis mass spectrometry (CE/MS) system has been developed in this work. A 100% electrical driven miniaturized CE device was integrated with a miniature MS instrument, which has a discontinuous atmospheric pressure interface (DAPI) for coupling with atmospheric pressure ionization sources. A nanoelectrospray ionization (nano-ESI) source was developed with a sheath liquid interface for coupling the miniature CE and the MS system. A systematic characterization and optimization of the analytical performance have been done. The analysis of isobaric peptides and avoiding charge competition effects in nano-ESI sources have been demonstrated.

UR - https://www.scopus.com/pages/publications/84923196956

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JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 4

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Development and characterizations of a miniature capillary electrophoresis mass spectrometry system

Abstract

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