TY - GEN
T1 - Developing Long Lifetime Quinacridone Derivative for Fluorescence Lifetime Imaging of Living Cells
AU - Wu, Tong
AU - Liu, Yifan
AU - Jing, Jing
N1 - Publisher Copyright:
© 2025 Copyright held by the owner/author(s).
PY - 2025/8/25
Y1 - 2025/8/25
N2 - Fluorescence lifetime imaging has become increasingly popular in bioimaging analysis, owing to its distinct advantages. It is independent of probe concentration and excitation power, effectively avoids autofluorescence interference in organisms, and can distinguish between spectrally similar fluorophores. In this study, we developed CMFL-Chol, a polarity-sensitive thermally activated delayed fluorescence (TADF) material with a quinacridone fluorophore core. The fluorescence lifetime of CMFL-Chol is 16.70 ns. To enhance its water solubility, we encapsulated CMFL-Chol using DSPE-PEG-MAL, synthesizing CMFL-Chol nanoparticles (NPs) with a fluorescence lifetime of 13.11 ns. Finally, we applied CMFL-Chol NPs to fluorescence lifetime imaging in cells. The results demonstrated that CMFL-Chol NPs hold great potential for analyzing cellular physiological activity processes.
AB - Fluorescence lifetime imaging has become increasingly popular in bioimaging analysis, owing to its distinct advantages. It is independent of probe concentration and excitation power, effectively avoids autofluorescence interference in organisms, and can distinguish between spectrally similar fluorophores. In this study, we developed CMFL-Chol, a polarity-sensitive thermally activated delayed fluorescence (TADF) material with a quinacridone fluorophore core. The fluorescence lifetime of CMFL-Chol is 16.70 ns. To enhance its water solubility, we encapsulated CMFL-Chol using DSPE-PEG-MAL, synthesizing CMFL-Chol nanoparticles (NPs) with a fluorescence lifetime of 13.11 ns. Finally, we applied CMFL-Chol NPs to fluorescence lifetime imaging in cells. The results demonstrated that CMFL-Chol NPs hold great potential for analyzing cellular physiological activity processes.
KW - Fluorescence Lifetime Imaging
KW - Nanoparticles
KW - Quinacridone Fluorophore
UR - https://www.scopus.com/pages/publications/105025195608
U2 - 10.1145/3747357.3747366
DO - 10.1145/3747357.3747366
M3 - Conference contribution
AN - SCOPUS:105025195608
T3 - Proceedings of 2025 International Symposium on Bioinformatics and Computational Biology, ISBCB 2025
SP - 54
EP - 61
BT - Proceedings of 2025 International Symposium on Bioinformatics and Computational Biology, ISBCB 2025
PB - Association for Computing Machinery, Inc
T2 - 2025 International Symposium on Bioinformatics and Computational Biology, ISBCB 2025
Y2 - 25 April 2025 through 27 April 2025
ER -