Determination of artemisinin in artemisinin tablets by HPLC-ELSD

Dong Zhang; Lan Yang; Man Yuan Wang; You You Tu

Determination of artemisinin in artemisinin tablets by HPLC-ELSD

Dong Zhang^*
, Lan Yang
, Man Yuan Wang
, You You Tu

^*Corresponding author for this work

China Academy of Chinese Medical Sciences
Capital Medical University

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

OBJECTIVE: To establish a HPLC-ELSD method for the determination of the content of artemisinin in artemisinin tablets. METHODS: The analytical column was Thermo RP-C₁₈ (4.6 mm x 250 mm,5 μm). The mobile phase was methanol-water (80:20). The flow rate was 1.0 mL·min^-1. The samples were determined by an evaporative light-scattering detector (ELSD) Waters 2420 with the drift tube temperature at 60°C. and the nitrogen flow pressure of 5.8 × 10^-3 Pa. RESULTS: The calibration curve was linear in the range of 1.002 - 4.008 μg (for artemisinin, r = 0.999 9). The average recovery (n = 5) was 99.45% (RSD = 2.3%). CONCLUSION: The method is simple, accurate and with good reproducibility. It can be used as the quality control of artemisinin tablet.

Original language	English
Pages (from-to)	491+492
Journal	Chinese Pharmaceutical Journal
Volume	43
Issue number	7
Publication status	Published - Apr 2008
Externally published	Yes

Keywords

Artemisinin
Artemisinin tablet
Determination
HPLC-ELSD

Cite this

@article{49980e3ad5cb472ebbd2edf7dfd4e305,

title = "Determination of artemisinin in artemisinin tablets by HPLC-ELSD",

abstract = "OBJECTIVE: To establish a HPLC-ELSD method for the determination of the content of artemisinin in artemisinin tablets. METHODS: The analytical column was Thermo RP-C18 (4.6 mm x 250 mm,5 μm). The mobile phase was methanol-water (80:20). The flow rate was 1.0 mL·min-1. The samples were determined by an evaporative light-scattering detector (ELSD) Waters 2420 with the drift tube temperature at 60°C. and the nitrogen flow pressure of 5.8 {\texttimes} 10-3 Pa. RESULTS: The calibration curve was linear in the range of 1.002 - 4.008 μg (for artemisinin, r = 0.999 9). The average recovery (n = 5) was 99.45\% (RSD = 2.3\%). CONCLUSION: The method is simple, accurate and with good reproducibility. It can be used as the quality control of artemisinin tablet.",

keywords = "Artemisinin, Artemisinin tablet, Determination, HPLC-ELSD",

author = "Dong Zhang and Lan Yang and Wang, \{Man Yuan\} and Tu, \{You You\}",

year = "2008",

month = apr,

language = "English",

volume = "43",

pages = "491+492",

journal = "Chinese Pharmaceutical Journal",

issn = "1001-2494",

publisher = "Chinese Pharmaceutical Association",

number = "7",

}

TY - JOUR

T1 - Determination of artemisinin in artemisinin tablets by HPLC-ELSD

AU - Zhang, Dong

AU - Yang, Lan

AU - Wang, Man Yuan

AU - Tu, You You

PY - 2008/4

Y1 - 2008/4

N2 - OBJECTIVE: To establish a HPLC-ELSD method for the determination of the content of artemisinin in artemisinin tablets. METHODS: The analytical column was Thermo RP-C18 (4.6 mm x 250 mm,5 μm). The mobile phase was methanol-water (80:20). The flow rate was 1.0 mL·min-1. The samples were determined by an evaporative light-scattering detector (ELSD) Waters 2420 with the drift tube temperature at 60°C. and the nitrogen flow pressure of 5.8 × 10-3 Pa. RESULTS: The calibration curve was linear in the range of 1.002 - 4.008 μg (for artemisinin, r = 0.999 9). The average recovery (n = 5) was 99.45% (RSD = 2.3%). CONCLUSION: The method is simple, accurate and with good reproducibility. It can be used as the quality control of artemisinin tablet.

AB - OBJECTIVE: To establish a HPLC-ELSD method for the determination of the content of artemisinin in artemisinin tablets. METHODS: The analytical column was Thermo RP-C18 (4.6 mm x 250 mm,5 μm). The mobile phase was methanol-water (80:20). The flow rate was 1.0 mL·min-1. The samples were determined by an evaporative light-scattering detector (ELSD) Waters 2420 with the drift tube temperature at 60°C. and the nitrogen flow pressure of 5.8 × 10-3 Pa. RESULTS: The calibration curve was linear in the range of 1.002 - 4.008 μg (for artemisinin, r = 0.999 9). The average recovery (n = 5) was 99.45% (RSD = 2.3%). CONCLUSION: The method is simple, accurate and with good reproducibility. It can be used as the quality control of artemisinin tablet.

KW - Artemisinin

KW - Artemisinin tablet

KW - Determination

KW - HPLC-ELSD

UR - https://www.scopus.com/pages/publications/52749085581

M3 - Article

AN - SCOPUS:52749085581

SN - 1001-2494

VL - 43

SP - 491+492

JO - Chinese Pharmaceutical Journal

JF - Chinese Pharmaceutical Journal

IS - 7

ER -

Determination of artemisinin in artemisinin tablets by HPLC-ELSD

Abstract

Keywords

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