Detection of the concentration of glucose solution using optical waveguide resonance technology combined with colorimetry

Glucose is a major component of the carbohydrates in the bodies of animals and plants, and it is very important to determine its quantitative in many areas. In this work, the colorimetric method combined with a double-sided metal coated waveguide was used to constitute a high sensitive oscillation field optical waveguide sensor. The glucose solution was colored by mixing with the oxidase reagent and then was injected into the waveguide. The attenuated total reflection(ATR) absorption peaks could be obtained through angle scanning, with the 650 nm laser beam incidenting on the surface of the waveguide. And the concentration changes of the glucose solution could be detected according to the minimum value of the absorption peaks. The results show that the resolution to the concentration of solution of this method can reach to 111 nmol/mL.