Culture and identification of neonate rat retinal microglia cells in vitro

Nan Wu; Yan Hua Wang; Dong Ning Liu; Yi Wang

Culture and identification of neonate rat retinal microglia cells in vitro

Nan Wu, Yan Hua Wang, Dong Ning Liu, Yi Wang^*

^*Corresponding author for this work

Army Medical University

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

• Aim: To establish a culture method of Long Evans neonate rat retinal microglia cells(RMGs) in vitro. • Methods: Mixed glial cells of Long Evans rats (postnatal <3d) were cultured for 10-12 days, then RMGs were purified by shaking in 37°C swing bed and identified by specific microglia marker IB-4 and CD11b, and cell surface of RMGs were observed by Scanning EM. • Results: Purity of RMGs were 94.5% and 95.8% by cell immunochemistry identification of IB-4 and CD11b, cell surface showed spinous processes of RMGs, contrasting the smooth surfaces of macrophages. • Conclusion: Based on these data, a method is presented that proposes to use the microglial cell to effectively deliver therapeutic compounds to retinal disease.

Original language	English
Pages (from-to)	1039-1041
Number of pages	3
Journal	International Journal of Ophthalmology
Volume	6
Issue number	5
Publication status	Published - Oct 2006
Externally published	Yes

Keywords

Cell culture
Long Evans rats
Retinal microglial cell

Cite this

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title = "Culture and identification of neonate rat retinal microglia cells in vitro",

abstract = "• Aim: To establish a culture method of Long Evans neonate rat retinal microglia cells(RMGs) in vitro. • Methods: Mixed glial cells of Long Evans rats (postnatal <3d) were cultured for 10-12 days, then RMGs were purified by shaking in 37°C swing bed and identified by specific microglia marker IB-4 and CD11b, and cell surface of RMGs were observed by Scanning EM. • Results: Purity of RMGs were 94.5% and 95.8% by cell immunochemistry identification of IB-4 and CD11b, cell surface showed spinous processes of RMGs, contrasting the smooth surfaces of macrophages. • Conclusion: Based on these data, a method is presented that proposes to use the microglial cell to effectively deliver therapeutic compounds to retinal disease.",

keywords = "Cell culture, Long Evans rats, Retinal microglial cell",

author = "Nan Wu and Wang, {Yan Hua} and Liu, {Dong Ning} and Yi Wang",

year = "2006",

month = oct,

language = "English",

volume = "6",

pages = "1039--1041",

journal = "International Journal of Ophthalmology",

issn = "2222-3959",

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TY - JOUR

T1 - Culture and identification of neonate rat retinal microglia cells in vitro

AU - Wu, Nan

AU - Wang, Yan Hua

AU - Liu, Dong Ning

AU - Wang, Yi

PY - 2006/10

Y1 - 2006/10

N2 - • Aim: To establish a culture method of Long Evans neonate rat retinal microglia cells(RMGs) in vitro. • Methods: Mixed glial cells of Long Evans rats (postnatal <3d) were cultured for 10-12 days, then RMGs were purified by shaking in 37°C swing bed and identified by specific microglia marker IB-4 and CD11b, and cell surface of RMGs were observed by Scanning EM. • Results: Purity of RMGs were 94.5% and 95.8% by cell immunochemistry identification of IB-4 and CD11b, cell surface showed spinous processes of RMGs, contrasting the smooth surfaces of macrophages. • Conclusion: Based on these data, a method is presented that proposes to use the microglial cell to effectively deliver therapeutic compounds to retinal disease.

AB - • Aim: To establish a culture method of Long Evans neonate rat retinal microglia cells(RMGs) in vitro. • Methods: Mixed glial cells of Long Evans rats (postnatal <3d) were cultured for 10-12 days, then RMGs were purified by shaking in 37°C swing bed and identified by specific microglia marker IB-4 and CD11b, and cell surface of RMGs were observed by Scanning EM. • Results: Purity of RMGs were 94.5% and 95.8% by cell immunochemistry identification of IB-4 and CD11b, cell surface showed spinous processes of RMGs, contrasting the smooth surfaces of macrophages. • Conclusion: Based on these data, a method is presented that proposes to use the microglial cell to effectively deliver therapeutic compounds to retinal disease.

KW - Cell culture

KW - Long Evans rats

KW - Retinal microglial cell

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M3 - Article

AN - SCOPUS:33751020250

SN - 2222-3959

VL - 6

SP - 1039

EP - 1041

JO - International Journal of Ophthalmology

JF - International Journal of Ophthalmology

IS - 5

ER -

Culture and identification of neonate rat retinal microglia cells in vitro

Abstract

Keywords

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