Cloning, expression and functioning of phlD genes from Pseudomonas fluorescens

The critical gene phlD for 2, 4-DAPG biosynthesis from Pseudomonas fluorescens was cloned and expressed in E. coli BL21(DE3)/pET28a(+)/phlD recombinant strain. The gene of phlD was amplified using Pseudomonas fluorescens 2P24 genome as template. The PhlD of 42000 Da fusion protein was induced and expressed in E. coli BL21(DE3)/pET28a(+) system. The phlD gene sequence was analyzed, the senior structure of the protein coded by the phlD also forecasted. PhlD was one of polyketide synthase of type-III, and the function of PhlD was similar to the polyketal synthase and acyltransferase. The fermentation liquid of recombinant strain was detected, and synthesis of the phloroglucinol was implemented.