Chemiluminescence Resonance Energy Transfer-Based Mesoporous Silica Nanosensors for the Detection of miRNA

The chemiluminescence resonance energy transfer (CRET)-based method is free of autofluorescence interference, which can achieve an extremely high signal-to-background ratio for detection. Nevertheless, this method is still hindered by the inner filter effect, quenching effect, and nonspecific absorption of reported nanoparticles. Herein, mesoporous silica nanomaterials (MSNs) acted as carriers to load both the donor (horseradish peroxidase, HRP) and the acceptor (a functional DNA duplex). This approach realized the construction of a new CRET-based nanosensor for the sensitive detection of miRNA. By controlling the energy-transfer distance with the designed DNAs, the donor emission at 430 nm could be quenched by the adsorption of the dye labeled on the acceptor DNA. The CRET system could be destroyed by releasing acceptor DNA from linker DNA via the competitive hybridization of target miRNA, resulting in emission recovery for quantification. With the cancer biomarker miR-155 as the model, the sensitive and selective detection of miR-155 was achieved, which showed high energy-transfer efficiency, good specificity, favorable biodegradability, and low toxicity. This work provides a potential pathway for biological detection and clinical diagnosis.