Cellular Delivery of siRNA Using Poly(2-dimethylaminoethyl methacrylate)- Functionalized Graphene Oxide Nano-Wrap

Jung Eun Lee; Kyuri Lee; Jeong A. Nam; Aesun Kim; So Young Lee; Min Sang Lee; Nak Won Kim; Yue Yin; Ji Won Park; Sung Young Park; Ji Hoon Jeong

doi:10.1007/s13233-019-7017-4

Cellular Delivery of siRNA Using Poly(2-dimethylaminoethyl methacrylate)- Functionalized Graphene Oxide Nano-Wrap

Jung Eun Lee, Kyuri Lee, Jeong A. Nam, Aesun Kim, So Young Lee, Min Sang Lee, Nak Won Kim, Yue Yin, Ji Won Park, Sung Young Park, Ji Hoon Jeong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Despite its high therapeutic potential, the use of siRNA has still been limited mostly due to the lack of a proper delivery system. Small number of charges and inflexible structure of siRNA are the major factors that affect cellular delivery efficiency of conventional lipid and polymeric carriers. In this study, we synthesized a 2-dimensional (2D) carrier for siRNA using a facile method to functionalize and stabilize the surface of graphene oxide (GO) with catechol-derivatized poly(2-dimethylaminoethyl methacrylate) (C-p(DMAEMA)). The resulting GO/C-p(DMAEMA) can interact with siRNA on the flexible 2D surface of the GO, wrapping up siRNA molecules by folding to form a dim sum-like nano structure. GO/C-p(DMAEMA) demonstrated significantly improved cellular uptake and enhanced gene silencing efficiency in the presence of serum protein, compared to unmodified GO and bPEI 25 kDa. [Figure not available: see fulltext.].

Original language	English
Pages (from-to)	1115-1122
Number of pages	8
Journal	Macromolecular Research
Volume	26
Issue number	12
DOIs	https://doi.org/10.1007/s13233-019-7017-4
Publication status	Published - 1 Dec 2018
Externally published	Yes

Keywords

dim sum-like structure
graphene oxide
nano-wrap
non-viral gene delivery
siRNA

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10.1007/s13233-019-7017-4

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@article{1d19a3e6b4ce43bab5f1427fd7f2b4df,

title = "Cellular Delivery of siRNA Using Poly(2-dimethylaminoethyl methacrylate)- Functionalized Graphene Oxide Nano-Wrap",

abstract = "Despite its high therapeutic potential, the use of siRNA has still been limited mostly due to the lack of a proper delivery system. Small number of charges and inflexible structure of siRNA are the major factors that affect cellular delivery efficiency of conventional lipid and polymeric carriers. In this study, we synthesized a 2-dimensional (2D) carrier for siRNA using a facile method to functionalize and stabilize the surface of graphene oxide (GO) with catechol-derivatized poly(2-dimethylaminoethyl methacrylate) (C-p(DMAEMA)). The resulting GO/C-p(DMAEMA) can interact with siRNA on the flexible 2D surface of the GO, wrapping up siRNA molecules by folding to form a dim sum-like nano structure. GO/C-p(DMAEMA) demonstrated significantly improved cellular uptake and enhanced gene silencing efficiency in the presence of serum protein, compared to unmodified GO and bPEI 25 kDa. [Figure not available: see fulltext.].",

keywords = "dim sum-like structure, graphene oxide, nano-wrap, non-viral gene delivery, siRNA",

author = "Lee, {Jung Eun} and Kyuri Lee and Nam, {Jeong A.} and Aesun Kim and Lee, {So Young} and Lee, {Min Sang} and Kim, {Nak Won} and Yue Yin and Park, {Ji Won} and Park, {Sung Young} and Jeong, {Ji Hoon}",

note = "Publisher Copyright: {\textcopyright} 2018, The Polymer Society of Korea and Springer Nature B.V.",

year = "2018",

month = dec,

day = "1",

doi = "10.1007/s13233-019-7017-4",

language = "English",

volume = "26",

pages = "1115--1122",

journal = "Macromolecular Research",

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TY - JOUR

T1 - Cellular Delivery of siRNA Using Poly(2-dimethylaminoethyl methacrylate)- Functionalized Graphene Oxide Nano-Wrap

AU - Lee, Jung Eun

AU - Lee, Kyuri

AU - Nam, Jeong A.

AU - Kim, Aesun

AU - Lee, So Young

AU - Lee, Min Sang

AU - Kim, Nak Won

AU - Yin, Yue

AU - Park, Ji Won

AU - Park, Sung Young

AU - Jeong, Ji Hoon

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Despite its high therapeutic potential, the use of siRNA has still been limited mostly due to the lack of a proper delivery system. Small number of charges and inflexible structure of siRNA are the major factors that affect cellular delivery efficiency of conventional lipid and polymeric carriers. In this study, we synthesized a 2-dimensional (2D) carrier for siRNA using a facile method to functionalize and stabilize the surface of graphene oxide (GO) with catechol-derivatized poly(2-dimethylaminoethyl methacrylate) (C-p(DMAEMA)). The resulting GO/C-p(DMAEMA) can interact with siRNA on the flexible 2D surface of the GO, wrapping up siRNA molecules by folding to form a dim sum-like nano structure. GO/C-p(DMAEMA) demonstrated significantly improved cellular uptake and enhanced gene silencing efficiency in the presence of serum protein, compared to unmodified GO and bPEI 25 kDa. [Figure not available: see fulltext.].

AB - Despite its high therapeutic potential, the use of siRNA has still been limited mostly due to the lack of a proper delivery system. Small number of charges and inflexible structure of siRNA are the major factors that affect cellular delivery efficiency of conventional lipid and polymeric carriers. In this study, we synthesized a 2-dimensional (2D) carrier for siRNA using a facile method to functionalize and stabilize the surface of graphene oxide (GO) with catechol-derivatized poly(2-dimethylaminoethyl methacrylate) (C-p(DMAEMA)). The resulting GO/C-p(DMAEMA) can interact with siRNA on the flexible 2D surface of the GO, wrapping up siRNA molecules by folding to form a dim sum-like nano structure. GO/C-p(DMAEMA) demonstrated significantly improved cellular uptake and enhanced gene silencing efficiency in the presence of serum protein, compared to unmodified GO and bPEI 25 kDa. [Figure not available: see fulltext.].

KW - dim sum-like structure

KW - graphene oxide

KW - nano-wrap

KW - non-viral gene delivery

KW - siRNA

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U2 - 10.1007/s13233-019-7017-4

DO - 10.1007/s13233-019-7017-4

M3 - Article

AN - SCOPUS:85056718804

SN - 1598-5032

VL - 26

SP - 1115

EP - 1122

JO - Macromolecular Research

JF - Macromolecular Research

IS - 12

ER -

Cellular Delivery of siRNA Using Poly(2-dimethylaminoethyl methacrylate)- Functionalized Graphene Oxide Nano-Wrap

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Keywords

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