Abstract
Biosynthesis of glycyrrhetinic acid monoglucuronide (GAMG) by β-D-glucuronidase from the whole cell of Penicillium purpurogenum Li-3 in an aqueous/organic system was studied. Results showed that tert-butyl alcohol is the best organic solvent and the optimum volume ratio of aqueous to organic phase is 3 to 7. The rest optimum conditions for this biocatalysis are 1.5 g·L-1 of substrate concentration, 45°C of temperature, pH 5.0, 7 g·L-1 of enzyme concentration and 120 r·min-1 of shaking rate. Under these optimized conditions, 79.12% yield of GAMG was achieved after 48 h reaction. The whole-cell enzyme has a good reusability and a high stability, which demonstrates the prospect for real application.
| Original language | English |
|---|---|
| Pages (from-to) | 2040-2045 |
| Number of pages | 6 |
| Journal | Huagong Xuebao/CIESC Journal |
| Volume | 60 |
| Issue number | 8 |
| Publication status | Published - Aug 2009 |
Keywords
- Aqueous/organic system
- Bioconversion
- Glycyrrhetinic acid monoglucuronide
- Penicillium purpurogenum
- Whole-cell
- β-D-glucuronidase