TY - JOUR
T1 - Bacillus oceanisediminis sp. nov., isolated from marine sediment
AU - Zhang, Jianli
AU - Wang, Jiewei
AU - Fang, Caiyuan
AU - Song, Fei
AU - Xin, Yuhua
AU - Qu, Lei
AU - Ding, Kai
PY - 2010/12
Y1 - 2010/12
N2 - A Gram-stain-positive, spore-forming, rod-shaped and aerobic bacterium was isolated from a sediment sample from the South Sea in China. The isolate, designated H2T, grew at 4-45 °C (optimum 37 °C) and pH 6-10 (optimum pH 7.0). The cell-wall peptidoglycan contained mesodiaminopimelic acid. The major isoprenoid quinone was MK-7 and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown aminophospholipid. The major fatty acid was iso-C15 : 0. The genomic DNA G+C content of strain H2T was 44.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a monophyletic clade with Bacillus firmus IAM 12464T. DNA-DNA relatedness between the isolate and B. firmus ATCC 14575T was low (27.5 %). Strain H2T also had a phenotypic profile that readily distinguished it from its closest phylogenetic neighbours. It is evident from the combination of genotypic and phenotypic data that the organism should be classified in a novel species of the genus Bacillus, for which the name Bacillus oceanisediminis sp. nov. is proposed. The type strain is H2T (=CGMCC 1.10115T =JCM 16506T).
AB - A Gram-stain-positive, spore-forming, rod-shaped and aerobic bacterium was isolated from a sediment sample from the South Sea in China. The isolate, designated H2T, grew at 4-45 °C (optimum 37 °C) and pH 6-10 (optimum pH 7.0). The cell-wall peptidoglycan contained mesodiaminopimelic acid. The major isoprenoid quinone was MK-7 and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown aminophospholipid. The major fatty acid was iso-C15 : 0. The genomic DNA G+C content of strain H2T was 44.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a monophyletic clade with Bacillus firmus IAM 12464T. DNA-DNA relatedness between the isolate and B. firmus ATCC 14575T was low (27.5 %). Strain H2T also had a phenotypic profile that readily distinguished it from its closest phylogenetic neighbours. It is evident from the combination of genotypic and phenotypic data that the organism should be classified in a novel species of the genus Bacillus, for which the name Bacillus oceanisediminis sp. nov. is proposed. The type strain is H2T (=CGMCC 1.10115T =JCM 16506T).
UR - http://www.scopus.com/inward/record.url?scp=78650224483&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.019851-0
DO - 10.1099/ijs.0.019851-0
M3 - Article
C2 - 20118297
AN - SCOPUS:78650224483
SN - 1466-5026
VL - 60
SP - 2924
EP - 2929
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 12
ER -