TY - JOUR
T1 - Bacillus nanhaiisediminis sp. nov., an alkalitolerant member of Bacillus rRNA group 6
AU - Zhang, Jianli
AU - Wang, Jiewei
AU - Song, Fei
AU - Fang, Caiyuan
AU - Xin, Yuhua
AU - Zhang, Yabo
PY - 2011/5
Y1 - 2011/5
N2 - A Gram-stain-positive, rod-shaped bacterium, designated strain NH3T, was isolated from a sediment sample from the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew optimally at 37 6° and pH 9. Strain NH3T had cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The cellular fatty acid profile included significant amounts of iso-C15: 0 and iso-C14: 0. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strain NH3T was 40.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain NH3T was a member of rRNA group 6 of the genus Bacillus, which includes alkalitolerant, alkaliphilic and halotolerant species. The closest phylogenetic relatives were Bacillus akibai 1139T (96.82% 16S rRNA gene sequence similarity), B. pseudofirmus DSM 8715T (96.76%), B. okhensis Kh10-101T (96.76%) and B. alkalidiazotrophicus MS 6T (96.47%). Strain NH3T could be distinguished from these phylogenetically close neighbours based on a number of phenotypic properties. On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, we conclude that strain NH3T (5CGMCC 1.10116T 5JCM 16507T) merits classification as the type strain of a novel species, for which the name Bacillus nanhaiisediminis sp. nov. is proposed.
AB - A Gram-stain-positive, rod-shaped bacterium, designated strain NH3T, was isolated from a sediment sample from the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew optimally at 37 6° and pH 9. Strain NH3T had cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The cellular fatty acid profile included significant amounts of iso-C15: 0 and iso-C14: 0. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strain NH3T was 40.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain NH3T was a member of rRNA group 6 of the genus Bacillus, which includes alkalitolerant, alkaliphilic and halotolerant species. The closest phylogenetic relatives were Bacillus akibai 1139T (96.82% 16S rRNA gene sequence similarity), B. pseudofirmus DSM 8715T (96.76%), B. okhensis Kh10-101T (96.76%) and B. alkalidiazotrophicus MS 6T (96.47%). Strain NH3T could be distinguished from these phylogenetically close neighbours based on a number of phenotypic properties. On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, we conclude that strain NH3T (5CGMCC 1.10116T 5JCM 16507T) merits classification as the type strain of a novel species, for which the name Bacillus nanhaiisediminis sp. nov. is proposed.
UR - https://www.scopus.com/pages/publications/79955829380
U2 - 10.1099/ijs.0.023671-0
DO - 10.1099/ijs.0.023671-0
M3 - Article
C2 - 20525818
AN - SCOPUS:79955829380
SN - 1466-5026
VL - 61
SP - 1078
EP - 1083
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 5
ER -