Automated Antithrombin Activity Detection with Whole Capillary Blood Based on Digital Microfluidic Platform

Dongshuo Li; Hanqi Hu; Hanzhi Zhang; Lei Shang; Tao Zhao; Qingchen Zhao; Shuhao Zhang; Fucun Ma; Guowei Liang; Rongxin Fu; Xuekai Liu

doi:10.3390/mi16070785

Automated Antithrombin Activity Detection with Whole Capillary Blood Based on Digital Microfluidic Platform

Dongshuo Li
, Hanqi Hu
, Hanzhi Zhang
, Lei Shang
, Tao Zhao
, Qingchen Zhao
, Shuhao Zhang
, Fucun Ma
, Guowei Liang^*
, Rongxin Fu^*
, Xuekai Liu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Antithrombin (AT) plays a crucial role in the human anticoagulant system and has extensive clinical applications. However, traditional detection methods often require large sample volumes, complex procedures, and lengthy processing times. Methods: We integrated digital microfluidics technology with AT detection to develop a point-of-care testing (POCT) device that is user-friendly and fully automated for real-time AT testing. Results: This device allows for automation and enhanced adaptability to various settings, requiring only a minimal sample volume (whole capillary blood), thereby omitting steps such as plasma separation to save time and improve clinical testing efficiency. Comparisons with conventional AT activity detection methods demonstrate a high degree of consistency in the results obtained with this device. Conclusion: The AT detection system we developed exhibits significant effectiveness and holds substantial research potential, positioning it to evolve into a clinically impactful POCT solution for AT assessment.

Original language	English
Article number	785
Journal	Micromachines
Volume	16
Issue number	7
DOIs	https://doi.org/10.3390/mi16070785
Publication status	Published - Jul 2025
Externally published	Yes

Keywords

AT
in vitro diagnostics
microfluidics
optical biosensors
point-of-care testing

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10.3390/mi16070785

Cite this

@article{67697895336341a1a3f3c48ef51c201d,

title = "Automated Antithrombin Activity Detection with Whole Capillary Blood Based on Digital Microfluidic Platform",

abstract = "Antithrombin (AT) plays a crucial role in the human anticoagulant system and has extensive clinical applications. However, traditional detection methods often require large sample volumes, complex procedures, and lengthy processing times. Methods: We integrated digital microfluidics technology with AT detection to develop a point-of-care testing (POCT) device that is user-friendly and fully automated for real-time AT testing. Results: This device allows for automation and enhanced adaptability to various settings, requiring only a minimal sample volume (whole capillary blood), thereby omitting steps such as plasma separation to save time and improve clinical testing efficiency. Comparisons with conventional AT activity detection methods demonstrate a high degree of consistency in the results obtained with this device. Conclusion: The AT detection system we developed exhibits significant effectiveness and holds substantial research potential, positioning it to evolve into a clinically impactful POCT solution for AT assessment.",

keywords = "AT, in vitro diagnostics, microfluidics, optical biosensors, point-of-care testing",

author = "Dongshuo Li and Hanqi Hu and Hanzhi Zhang and Lei Shang and Tao Zhao and Qingchen Zhao and Shuhao Zhang and Fucun Ma and Guowei Liang and Rongxin Fu and Xuekai Liu",

note = "Publisher Copyright: {\textcopyright} 2025 by the authors.",

year = "2025",

month = jul,

doi = "10.3390/mi16070785",

language = "English",

volume = "16",

journal = "Micromachines",

issn = "2072-666X",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "7",

}

TY - JOUR

T1 - Automated Antithrombin Activity Detection with Whole Capillary Blood Based on Digital Microfluidic Platform

AU - Li, Dongshuo

AU - Hu, Hanqi

AU - Zhang, Hanzhi

AU - Shang, Lei

AU - Zhao, Tao

AU - Zhao, Qingchen

AU - Zhang, Shuhao

AU - Ma, Fucun

AU - Liang, Guowei

AU - Fu, Rongxin

AU - Liu, Xuekai

PY - 2025/7

Y1 - 2025/7

N2 - Antithrombin (AT) plays a crucial role in the human anticoagulant system and has extensive clinical applications. However, traditional detection methods often require large sample volumes, complex procedures, and lengthy processing times. Methods: We integrated digital microfluidics technology with AT detection to develop a point-of-care testing (POCT) device that is user-friendly and fully automated for real-time AT testing. Results: This device allows for automation and enhanced adaptability to various settings, requiring only a minimal sample volume (whole capillary blood), thereby omitting steps such as plasma separation to save time and improve clinical testing efficiency. Comparisons with conventional AT activity detection methods demonstrate a high degree of consistency in the results obtained with this device. Conclusion: The AT detection system we developed exhibits significant effectiveness and holds substantial research potential, positioning it to evolve into a clinically impactful POCT solution for AT assessment.

AB - Antithrombin (AT) plays a crucial role in the human anticoagulant system and has extensive clinical applications. However, traditional detection methods often require large sample volumes, complex procedures, and lengthy processing times. Methods: We integrated digital microfluidics technology with AT detection to develop a point-of-care testing (POCT) device that is user-friendly and fully automated for real-time AT testing. Results: This device allows for automation and enhanced adaptability to various settings, requiring only a minimal sample volume (whole capillary blood), thereby omitting steps such as plasma separation to save time and improve clinical testing efficiency. Comparisons with conventional AT activity detection methods demonstrate a high degree of consistency in the results obtained with this device. Conclusion: The AT detection system we developed exhibits significant effectiveness and holds substantial research potential, positioning it to evolve into a clinically impactful POCT solution for AT assessment.

KW - AT

KW - in vitro diagnostics

KW - microfluidics

KW - optical biosensors

KW - point-of-care testing

UR - https://www.scopus.com/pages/publications/105011601422

U2 - 10.3390/mi16070785

DO - 10.3390/mi16070785

M3 - Article

AN - SCOPUS:105011601422

SN - 2072-666X

VL - 16

JO - Micromachines

JF - Micromachines

IS - 7

M1 - 785

ER -

Automated Antithrombin Activity Detection with Whole Capillary Blood Based on Digital Microfluidic Platform

Abstract

Keywords

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