Analysis of alkaloids in Nelumbo nucifera leaves by solid phase extraction-rapid resolution liquid chromatography-quadrupole-time of flight mass spectrometry

Zhen Jia Zheng; Xiao Wang; Ming Lin Wang; Shan Shan Wang; Xian En Zhao

Analysis of alkaloids in Nelumbo nucifera leaves by solid phase extraction-rapid resolution liquid chromatography-quadrupole-time of flight mass spectrometry

Zhen Jia Zheng^*
, Xiao Wang
, Ming Lin Wang
, Shan Shan Wang
, Xian En Zhao

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Objective: A novel method for the analysis of alkaloids in Nelumbo nucifera leaves was established by SPE-RRLC-Q-TOF mass spectrometry. Methods: The crude sample was extracted by 1% HCl with ultrasound-assisted extraction, then purified by SPE column, and eluted with ammonia-methanol (5:95). After concentration, the residue was dissolved by methanol solution. The real sample was analyzed by RRLC-Q-TOF. A Welch Materials C₁₈ column was applied in the RRLC separation using acetonitrile and water as mobile phase. The elutes were detected by Q-TOF to obtain the MS spectra with extract molecular weights under positive ion mode. Results: Nine alkaloids were identified. Conclusion: This method can be used to rapidly determine the alkaloids of N. nucifera leaves.

Original language	English
Pages (from-to)	1066-1068
Number of pages	3
Journal	Chinese Traditional and Herbal Drugs
Volume	42
Issue number	6
Publication status	Published - 12 Jun 2011
Externally published	Yes

Keywords

Alkaloids
Cation exchange
Nelumbo nucifera Gaertn. leaves
Rapid resolution liquid chromatography- quadrupole-time of flight (RRLC-Q-TOF) mass spectrometry
Solid phase extraction (SPE)

Cite this

@article{27431c7c2e59494f9059db679e4142bb,

title = "Analysis of alkaloids in Nelumbo nucifera leaves by solid phase extraction-rapid resolution liquid chromatography-quadrupole-time of flight mass spectrometry",

abstract = "Objective: A novel method for the analysis of alkaloids in Nelumbo nucifera leaves was established by SPE-RRLC-Q-TOF mass spectrometry. Methods: The crude sample was extracted by 1\% HCl with ultrasound-assisted extraction, then purified by SPE column, and eluted with ammonia-methanol (5:95). After concentration, the residue was dissolved by methanol solution. The real sample was analyzed by RRLC-Q-TOF. A Welch Materials C18 column was applied in the RRLC separation using acetonitrile and water as mobile phase. The elutes were detected by Q-TOF to obtain the MS spectra with extract molecular weights under positive ion mode. Results: Nine alkaloids were identified. Conclusion: This method can be used to rapidly determine the alkaloids of N. nucifera leaves.",

keywords = "Alkaloids, Cation exchange, Nelumbo nucifera Gaertn. leaves, Rapid resolution liquid chromatography- quadrupole-time of flight (RRLC-Q-TOF) mass spectrometry, Solid phase extraction (SPE)",

author = "Zheng, \{Zhen Jia\} and Xiao Wang and Wang, \{Ming Lin\} and Wang, \{Shan Shan\} and Zhao, \{Xian En\}",

year = "2011",

month = jun,

day = "12",

language = "English",

volume = "42",

pages = "1066--1068",

journal = "Chinese Traditional and Herbal Drugs",

issn = "0253-2670",

publisher = "Editorial Office of Chinese Traditional and Herbal Drugs",

number = "6",

}

TY - JOUR

T1 - Analysis of alkaloids in Nelumbo nucifera leaves by solid phase extraction-rapid resolution liquid chromatography-quadrupole-time of flight mass spectrometry

AU - Zheng, Zhen Jia

AU - Wang, Xiao

AU - Wang, Ming Lin

AU - Wang, Shan Shan

AU - Zhao, Xian En

PY - 2011/6/12

Y1 - 2011/6/12

N2 - Objective: A novel method for the analysis of alkaloids in Nelumbo nucifera leaves was established by SPE-RRLC-Q-TOF mass spectrometry. Methods: The crude sample was extracted by 1% HCl with ultrasound-assisted extraction, then purified by SPE column, and eluted with ammonia-methanol (5:95). After concentration, the residue was dissolved by methanol solution. The real sample was analyzed by RRLC-Q-TOF. A Welch Materials C18 column was applied in the RRLC separation using acetonitrile and water as mobile phase. The elutes were detected by Q-TOF to obtain the MS spectra with extract molecular weights under positive ion mode. Results: Nine alkaloids were identified. Conclusion: This method can be used to rapidly determine the alkaloids of N. nucifera leaves.

AB - Objective: A novel method for the analysis of alkaloids in Nelumbo nucifera leaves was established by SPE-RRLC-Q-TOF mass spectrometry. Methods: The crude sample was extracted by 1% HCl with ultrasound-assisted extraction, then purified by SPE column, and eluted with ammonia-methanol (5:95). After concentration, the residue was dissolved by methanol solution. The real sample was analyzed by RRLC-Q-TOF. A Welch Materials C18 column was applied in the RRLC separation using acetonitrile and water as mobile phase. The elutes were detected by Q-TOF to obtain the MS spectra with extract molecular weights under positive ion mode. Results: Nine alkaloids were identified. Conclusion: This method can be used to rapidly determine the alkaloids of N. nucifera leaves.

KW - Alkaloids

KW - Cation exchange

KW - Nelumbo nucifera Gaertn. leaves

KW - Rapid resolution liquid chromatography- quadrupole-time of flight (RRLC-Q-TOF) mass spectrometry

KW - Solid phase extraction (SPE)

UR - https://www.scopus.com/pages/publications/79960095213

M3 - Article

AN - SCOPUS:79960095213

SN - 0253-2670

VL - 42

SP - 1066

EP - 1068

JO - Chinese Traditional and Herbal Drugs

JF - Chinese Traditional and Herbal Drugs

IS - 6

ER -

Analysis of alkaloids in Nelumbo nucifera leaves by solid phase extraction-rapid resolution liquid chromatography-quadrupole-time of flight mass spectrometry

Abstract

Keywords

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