Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly

Juan Li; Xinrong Liu; Baoqing Zhu; Yingya Shao; Dongxu Zhao; Xinlin Yang

doi:10.1109/ICBBE.2008.684

Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly

Juan Li, Xinrong Liu, Baoqing Zhu, Yingya Shao, Dongxu Zhao, Xinlin Yang^*

^*Corresponding author for this work

School of Life Science

Beijing Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

6 Citations (Scopus)

Abstract

Electrostatic layer-by-layer self-assembly is a simple and effective approach to carry macromolecules into microscale carriers. Calcium-cross-linked alginate hydrogel microspheres were fabricated by an emulsification technique and then used as negatively charged templates for polyelectrolyte multilayer coatings to entrap heparin. The polycation was poly (allylamine hydrochloride) (PAH), while the polyanion was heparin, an anticoagulant to prevent the clotting of blood. The microcapsule was formed via coating {PAH/heparin}₃ on Ca²⁺-cross-linked alginate hydrogel. The retention of alginate in {PAH/heparin}₃ microcapsule was confirmed by fluorescence inverted microscopy and confocal laser scanning microscopy. The assembly of polyelectrolyte layers on alginate microspheres was monitored by Zeta Potential Analyzer. The concentration of heparin was detected by HPLC with IR-detector and organic acid column. It was found that heparin could be entrapped in the microcapsules in high efficiency, providing a useful delivery system which would be found potential application in antagonizing thrombosis.

Original language	English
Title of host publication	2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008
Publisher	IEEE Computer Society
Pages	1420-1423
Number of pages	4
ISBN (Print)	9781424417483
DOIs	https://doi.org/10.1109/ICBBE.2008.684
Publication status	Published - 2008
Event	2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008 - Shanghai, China Duration: 16 May 2008 → 18 May 2008

Publication series

Name	2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008

Conference

Conference	2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008
Country/Territory	China
City	Shanghai
Period	16/05/08 → 18/05/08

Keywords

Alginate
Heparin
Layer-by-Layer self-assembly
Microcapsules

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10.1109/ICBBE.2008.684

Cite this

Li, J., Liu, X., Zhu, B., Shao, Y., Zhao, D., & Yang, X. (2008). Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly. In 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008 (pp. 1420-1423). Article 4535564 (2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008). IEEE Computer Society. https://doi.org/10.1109/ICBBE.2008.684

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title = "Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly",

abstract = "Electrostatic layer-by-layer self-assembly is a simple and effective approach to carry macromolecules into microscale carriers. Calcium-cross-linked alginate hydrogel microspheres were fabricated by an emulsification technique and then used as negatively charged templates for polyelectrolyte multilayer coatings to entrap heparin. The polycation was poly (allylamine hydrochloride) (PAH), while the polyanion was heparin, an anticoagulant to prevent the clotting of blood. The microcapsule was formed via coating {PAH/heparin}3 on Ca2+-cross-linked alginate hydrogel. The retention of alginate in {PAH/heparin}3 microcapsule was confirmed by fluorescence inverted microscopy and confocal laser scanning microscopy. The assembly of polyelectrolyte layers on alginate microspheres was monitored by Zeta Potential Analyzer. The concentration of heparin was detected by HPLC with IR-detector and organic acid column. It was found that heparin could be entrapped in the microcapsules in high efficiency, providing a useful delivery system which would be found potential application in antagonizing thrombosis.",

keywords = "Alginate, Heparin, Layer-by-Layer self-assembly, Microcapsules",

author = "Juan Li and Xinrong Liu and Baoqing Zhu and Yingya Shao and Dongxu Zhao and Xinlin Yang",

year = "2008",

doi = "10.1109/ICBBE.2008.684",

language = "English",

isbn = "9781424417483",

series = "2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008",

publisher = "IEEE Computer Society",

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booktitle = "2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008",

address = "United States",

note = "2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008 ; Conference date: 16-05-2008 Through 18-05-2008",

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Li, J, Liu, X, Zhu, B, Shao, Y, Zhao, D & Yang, X 2008, Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly. in 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008., 4535564, 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008, IEEE Computer Society, pp. 1420-1423, 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008, Shanghai, China, 16/05/08. https://doi.org/10.1109/ICBBE.2008.684

Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly. / Li, Juan; Liu, Xinrong; Zhu, Baoqing et al.
2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008. IEEE Computer Society, 2008. p. 1420-1423 4535564 (2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly

AU - Li, Juan

AU - Liu, Xinrong

AU - Zhu, Baoqing

AU - Shao, Yingya

AU - Zhao, Dongxu

AU - Yang, Xinlin

PY - 2008

Y1 - 2008

N2 - Electrostatic layer-by-layer self-assembly is a simple and effective approach to carry macromolecules into microscale carriers. Calcium-cross-linked alginate hydrogel microspheres were fabricated by an emulsification technique and then used as negatively charged templates for polyelectrolyte multilayer coatings to entrap heparin. The polycation was poly (allylamine hydrochloride) (PAH), while the polyanion was heparin, an anticoagulant to prevent the clotting of blood. The microcapsule was formed via coating {PAH/heparin}3 on Ca2+-cross-linked alginate hydrogel. The retention of alginate in {PAH/heparin}3 microcapsule was confirmed by fluorescence inverted microscopy and confocal laser scanning microscopy. The assembly of polyelectrolyte layers on alginate microspheres was monitored by Zeta Potential Analyzer. The concentration of heparin was detected by HPLC with IR-detector and organic acid column. It was found that heparin could be entrapped in the microcapsules in high efficiency, providing a useful delivery system which would be found potential application in antagonizing thrombosis.

AB - Electrostatic layer-by-layer self-assembly is a simple and effective approach to carry macromolecules into microscale carriers. Calcium-cross-linked alginate hydrogel microspheres were fabricated by an emulsification technique and then used as negatively charged templates for polyelectrolyte multilayer coatings to entrap heparin. The polycation was poly (allylamine hydrochloride) (PAH), while the polyanion was heparin, an anticoagulant to prevent the clotting of blood. The microcapsule was formed via coating {PAH/heparin}3 on Ca2+-cross-linked alginate hydrogel. The retention of alginate in {PAH/heparin}3 microcapsule was confirmed by fluorescence inverted microscopy and confocal laser scanning microscopy. The assembly of polyelectrolyte layers on alginate microspheres was monitored by Zeta Potential Analyzer. The concentration of heparin was detected by HPLC with IR-detector and organic acid column. It was found that heparin could be entrapped in the microcapsules in high efficiency, providing a useful delivery system which would be found potential application in antagonizing thrombosis.

KW - Alginate

KW - Heparin

KW - Layer-by-Layer self-assembly

KW - Microcapsules

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SN - 9781424417483

T3 - 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008

SP - 1420

EP - 1423

BT - 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008

PB - IEEE Computer Society

T2 - 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008

Y2 - 16 May 2008 through 18 May 2008

ER -

Li J, Liu X, Zhu B, Shao Y, Zhao D, Yang X. Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly. In 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008. IEEE Computer Society. 2008. p. 1420-1423. 4535564. (2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008). doi: 10.1109/ICBBE.2008.684

Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly

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Keywords

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