A sensitive ratiometric fluorescent probe for quantitive detection and imaging of alkaline phosphatase in living cells

Congcong Gao; Shunping Zang; Longxue Nie; Yong Tian; Rubo Zhang; Jing Jing; Xiaoling Zhang

doi:10.1016/j.aca.2019.03.043

A sensitive ratiometric fluorescent probe for quantitive detection and imaging of alkaline phosphatase in living cells

Congcong Gao
, Shunping Zang
, Longxue Nie
, Yong Tian
, Rubo Zhang
, Jing Jing
, Xiaoling Zhang^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

We report a sensitive fluorescence probe Ratio-ALP for in vivo ratiometric imaging of alkaline phosphatase (ALP). The probe was designed by conjugating a naphthalimide with a phosphate group as the responsive moiety for enzymatic reactions. The fluorescence intensity ratio displayed a linear relationship against the concentration of ALP in the concentration range from 0 to 200 U/L, its LOD is as low as 0.25 U/L. Ratio-ALP has good selectivity and sensitivity, high cell permeability, and low cytotoxicity. Furthermore, probe Ratio-ALP was also successfully applied to image endogenous ALP activity in living cells.

Original language	English
Pages (from-to)	131-135
Number of pages	5
Journal	Analytica Chimica Acta
Volume	1066
DOIs	https://doi.org/10.1016/j.aca.2019.03.043
Publication status	Published - 20 Aug 2019

Keywords

Alkaline phosphatase
Fluorescent probe
Ratiometric

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10.1016/j.aca.2019.03.043

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title = "A sensitive ratiometric fluorescent probe for quantitive detection and imaging of alkaline phosphatase in living cells",

abstract = "We report a sensitive fluorescence probe Ratio-ALP for in vivo ratiometric imaging of alkaline phosphatase (ALP). The probe was designed by conjugating a naphthalimide with a phosphate group as the responsive moiety for enzymatic reactions. The fluorescence intensity ratio displayed a linear relationship against the concentration of ALP in the concentration range from 0 to 200 U/L, its LOD is as low as 0.25 U/L. Ratio-ALP has good selectivity and sensitivity, high cell permeability, and low cytotoxicity. Furthermore, probe Ratio-ALP was also successfully applied to image endogenous ALP activity in living cells.",

keywords = "Alkaline phosphatase, Fluorescent probe, Ratiometric",

author = "Congcong Gao and Shunping Zang and Longxue Nie and Yong Tian and Rubo Zhang and Jing Jing and Xiaoling Zhang",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

year = "2019",

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doi = "10.1016/j.aca.2019.03.043",

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T1 - A sensitive ratiometric fluorescent probe for quantitive detection and imaging of alkaline phosphatase in living cells

AU - Gao, Congcong

AU - Zang, Shunping

AU - Nie, Longxue

AU - Tian, Yong

AU - Zhang, Rubo

AU - Jing, Jing

AU - Zhang, Xiaoling

PY - 2019/8/20

Y1 - 2019/8/20

N2 - We report a sensitive fluorescence probe Ratio-ALP for in vivo ratiometric imaging of alkaline phosphatase (ALP). The probe was designed by conjugating a naphthalimide with a phosphate group as the responsive moiety for enzymatic reactions. The fluorescence intensity ratio displayed a linear relationship against the concentration of ALP in the concentration range from 0 to 200 U/L, its LOD is as low as 0.25 U/L. Ratio-ALP has good selectivity and sensitivity, high cell permeability, and low cytotoxicity. Furthermore, probe Ratio-ALP was also successfully applied to image endogenous ALP activity in living cells.

AB - We report a sensitive fluorescence probe Ratio-ALP for in vivo ratiometric imaging of alkaline phosphatase (ALP). The probe was designed by conjugating a naphthalimide with a phosphate group as the responsive moiety for enzymatic reactions. The fluorescence intensity ratio displayed a linear relationship against the concentration of ALP in the concentration range from 0 to 200 U/L, its LOD is as low as 0.25 U/L. Ratio-ALP has good selectivity and sensitivity, high cell permeability, and low cytotoxicity. Furthermore, probe Ratio-ALP was also successfully applied to image endogenous ALP activity in living cells.

KW - Alkaline phosphatase

KW - Fluorescent probe

KW - Ratiometric

UR - https://www.scopus.com/pages/publications/85063679887

U2 - 10.1016/j.aca.2019.03.043

DO - 10.1016/j.aca.2019.03.043

M3 - Article

C2 - 31027528

AN - SCOPUS:85063679887

SN - 0003-2670

VL - 1066

SP - 131

EP - 135

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

ER -

A sensitive ratiometric fluorescent probe for quantitive detection and imaging of alkaline phosphatase in living cells

Abstract

Keywords

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