A real-time ratiometric fluorescent probe for imaging of SO2 derivatives in mitochondria of living cells

Junwei Shi; Wei Shu; Yong Tian; Yulong Wu; Jing Jing; Rubo Zhang; Xiaoling Zhang

doi:10.1039/c9ra03207j

A real-time ratiometric fluorescent probe for imaging of SO₂ derivatives in mitochondria of living cells

Junwei Shi
, Wei Shu
, Yong Tian
, Yulong Wu
, Jing Jing
, Rubo Zhang
, Xiaoling Zhang

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A real-time ratiometric fluorescent probe (IN-CZ) for highly selective detection of sulfite was designed and synthesized, which is based on modulating the intramolecular charge transfer (ICT) of the hemicyanine dye platform. The mechanism of using the probe is mainly through the Michael addition that occurs between IN-CZ and sulfite with a detection limit of 2.99 × 10^-5 M. IN-CZ displays a fast response (within 1 minute) and is highly selective for SO₃^2-/HSO₃^- over ROS, biologically relevant ions, biological mercaptans and other reactive species. More importantly, IN-CZ was suitable for ratiometric fluorescence imaging in living cells, by real-time monitoring of SO₃^2-/HSO₃^- changes in mitochondria targeted in living cells.

Original language	English
Pages (from-to)	22348-22354
Number of pages	7
Journal	RSC Advances
Volume	9
Issue number	39
DOIs	https://doi.org/10.1039/c9ra03207j
Publication status	Published - 2019
Externally published	Yes

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title = "A real-time ratiometric fluorescent probe for imaging of SO2 derivatives in mitochondria of living cells",

abstract = "A real-time ratiometric fluorescent probe (IN-CZ) for highly selective detection of sulfite was designed and synthesized, which is based on modulating the intramolecular charge transfer (ICT) of the hemicyanine dye platform. The mechanism of using the probe is mainly through the Michael addition that occurs between IN-CZ and sulfite with a detection limit of 2.99 × 10-5 M. IN-CZ displays a fast response (within 1 minute) and is highly selective for SO32-/HSO3- over ROS, biologically relevant ions, biological mercaptans and other reactive species. More importantly, IN-CZ was suitable for ratiometric fluorescence imaging in living cells, by real-time monitoring of SO32-/HSO3- changes in mitochondria targeted in living cells.",

author = "Junwei Shi and Wei Shu and Yong Tian and Yulong Wu and Jing Jing and Rubo Zhang and Xiaoling Zhang",

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T1 - A real-time ratiometric fluorescent probe for imaging of SO2 derivatives in mitochondria of living cells

AU - Shi, Junwei

AU - Shu, Wei

AU - Tian, Yong

AU - Wu, Yulong

AU - Jing, Jing

AU - Zhang, Rubo

AU - Zhang, Xiaoling

PY - 2019

Y1 - 2019

N2 - A real-time ratiometric fluorescent probe (IN-CZ) for highly selective detection of sulfite was designed and synthesized, which is based on modulating the intramolecular charge transfer (ICT) of the hemicyanine dye platform. The mechanism of using the probe is mainly through the Michael addition that occurs between IN-CZ and sulfite with a detection limit of 2.99 × 10-5 M. IN-CZ displays a fast response (within 1 minute) and is highly selective for SO32-/HSO3- over ROS, biologically relevant ions, biological mercaptans and other reactive species. More importantly, IN-CZ was suitable for ratiometric fluorescence imaging in living cells, by real-time monitoring of SO32-/HSO3- changes in mitochondria targeted in living cells.

AB - A real-time ratiometric fluorescent probe (IN-CZ) for highly selective detection of sulfite was designed and synthesized, which is based on modulating the intramolecular charge transfer (ICT) of the hemicyanine dye platform. The mechanism of using the probe is mainly through the Michael addition that occurs between IN-CZ and sulfite with a detection limit of 2.99 × 10-5 M. IN-CZ displays a fast response (within 1 minute) and is highly selective for SO32-/HSO3- over ROS, biologically relevant ions, biological mercaptans and other reactive species. More importantly, IN-CZ was suitable for ratiometric fluorescence imaging in living cells, by real-time monitoring of SO32-/HSO3- changes in mitochondria targeted in living cells.

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DO - 10.1039/c9ra03207j

M3 - Article

AN - SCOPUS:85069791956

SN - 2046-2069

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SP - 22348

EP - 22354

JO - RSC Advances

JF - RSC Advances

IS - 39

ER -

A real-time ratiometric fluorescent probe for imaging of SO₂ derivatives in mitochondria of living cells

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