A novel pathway construction in Candida tropicalis for direct xylitol conversion from corncob xylan

In this study, an integrated xylitol production pathway, directly using xylan as the substrate, was constructed in Candida tropicalis BIT-Xol-1 which could efficiently convert xylose into xylitol. In order to consolidate this bioprocessing, a β-1,4-xylanase gene (atn) and a β-xylosidase gene (atl) were cloned from Aspergillus terreus, and were constructed onto episomal plasmid pAUR123. Additionally, combination of the individual atn and atl expression cassette was also cloned onto pAUR123. After transforming, the positive C. tropicalis transformants co-expressing xylanase and xylosidase produced larger hydrolysis zones than those expressing xylanase alone, when incubated on xylan-congo red plates. The engineered C. tropicalis/pAUR-. atn-. atl-3 (C. tropicalis PNL3) secrete heterologous xylanase and xylosidase simultaneously, with the activities of 48.17 and 11.56. U/mL, respectively. The xylitol yields by C. tropicalis PNL3 utilizing xylan and corncob were 77.1% and 66.9%, respectively. The integrated pathway of xylitol production was feasible and efficient in utilization of xylan-rich renewable biomass via combining saccharification and transformation of xylan in engineered C. tropicalis.