TY - JOUR
T1 - A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance
AU - Liu, Jingwen
AU - Yu, Yang
AU - Li, Fuhua
AU - Zhang, Xiaojun
AU - Xiang, Jianhai
N1 - Publisher Copyright:
© 2014, Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg.
PY - 2014/11
Y1 - 2014/11
N2 - Anti-lipopolysaccharide factors (ALFs) are basic components of the crustacean immune system that defend against a range of pathogens. The cDNA sequence of a new ALF, designated nLvALF2, with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the conserved functional domain of ALFs, the LPS binding domain (LBD). Its genomic sequence consisted of three exons and four introns. nLvALF2 was mainly expressed in the Oka organ and gills of shrimps. The transcriptional level of nLvALF2 increased significantly after white spot syndrome virus (WSSV) infection, suggesting its important roles in protecting shrimps from WSSV. Single nucleotide polymorphisms (SNPs) were found in the genomic sequence of nLvALF2, of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV. The loci g.2422 A>G, g.2466 T>C, and g.2529 G>A were significantly associated with the resistance to WSSV (P<0.05). These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei.
AB - Anti-lipopolysaccharide factors (ALFs) are basic components of the crustacean immune system that defend against a range of pathogens. The cDNA sequence of a new ALF, designated nLvALF2, with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the conserved functional domain of ALFs, the LPS binding domain (LBD). Its genomic sequence consisted of three exons and four introns. nLvALF2 was mainly expressed in the Oka organ and gills of shrimps. The transcriptional level of nLvALF2 increased significantly after white spot syndrome virus (WSSV) infection, suggesting its important roles in protecting shrimps from WSSV. Single nucleotide polymorphisms (SNPs) were found in the genomic sequence of nLvALF2, of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV. The loci g.2422 A>G, g.2466 T>C, and g.2529 G>A were significantly associated with the resistance to WSSV (P<0.05). These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei.
KW - anti-lipopolysaccharide factors (ALFs)
KW - Litopenaeus vannamei
KW - single nucleotide polymorphisms (SNPs)
KW - white spot syndrome virus (WSSV) resistance
UR - http://www.scopus.com/inward/record.url?scp=84919950685&partnerID=8YFLogxK
U2 - 10.1007/s00343-015-4010-4
DO - 10.1007/s00343-015-4010-4
M3 - Article
AN - SCOPUS:84919950685
SN - 0254-4059
VL - 32
SP - 1232
EP - 1247
JO - Chinese Journal of Oceanology and Limnology
JF - Chinese Journal of Oceanology and Limnology
IS - 6
ER -