A membrane-permeable dye for living cells with large two-photon excited fluorescence action cross-sections for bioimaging

Ruiqing Feng; Yuming Sun; Minggang Tian; Ge Zhang; Ruoyao Zhang; Lifang Guo; Xuechen Li; Xiaoqiang Yu; Ning Zhao

doi:10.1039/c5tb00940e

A membrane-permeable dye for living cells with large two-photon excited fluorescence action cross-sections for bioimaging

Ruiqing Feng, Yuming Sun, Minggang Tian, Ge Zhang, Ruoyao Zhang, Lifang Guo, Xuechen Li, Xiaoqiang Yu^*, Ning Zhao

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The development of two-photon fluorophores remains an important issue. Dyes that possess both large two-photon excited fluorescence action cross-sections and cell membrane permeability are especially in demand to maximize the underlying virtue of two-photon microscopy for bioimaging. Herein, a novel two-photon excited fluorescence dye has been synthesized. This V-shaped dye exhibited large two-photon excited fluorescence action cross-sections and high plasma membrane permeability. Cell imaging experiments demonstrated that the dye could stain living cells with bright two-photon excited fluorescence. All the results have indicated the potential of the dye as a basic platform for the development of two-photon excited fluorescence probes.

Original language	English
Pages (from-to)	8644-8649
Number of pages	6
Journal	Journal of Materials Chemistry B
Volume	3
Issue number	44
DOIs	https://doi.org/10.1039/c5tb00940e
Publication status	Published - 2015
Externally published	Yes

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title = "A membrane-permeable dye for living cells with large two-photon excited fluorescence action cross-sections for bioimaging",

abstract = "The development of two-photon fluorophores remains an important issue. Dyes that possess both large two-photon excited fluorescence action cross-sections and cell membrane permeability are especially in demand to maximize the underlying virtue of two-photon microscopy for bioimaging. Herein, a novel two-photon excited fluorescence dye has been synthesized. This V-shaped dye exhibited large two-photon excited fluorescence action cross-sections and high plasma membrane permeability. Cell imaging experiments demonstrated that the dye could stain living cells with bright two-photon excited fluorescence. All the results have indicated the potential of the dye as a basic platform for the development of two-photon excited fluorescence probes.",

author = "Ruiqing Feng and Yuming Sun and Minggang Tian and Ge Zhang and Ruoyao Zhang and Lifang Guo and Xuechen Li and Xiaoqiang Yu and Ning Zhao",

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year = "2015",

doi = "10.1039/c5tb00940e",

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T1 - A membrane-permeable dye for living cells with large two-photon excited fluorescence action cross-sections for bioimaging

AU - Feng, Ruiqing

AU - Sun, Yuming

AU - Tian, Minggang

AU - Zhang, Ge

AU - Zhang, Ruoyao

AU - Guo, Lifang

AU - Li, Xuechen

AU - Yu, Xiaoqiang

AU - Zhao, Ning

PY - 2015

Y1 - 2015

N2 - The development of two-photon fluorophores remains an important issue. Dyes that possess both large two-photon excited fluorescence action cross-sections and cell membrane permeability are especially in demand to maximize the underlying virtue of two-photon microscopy for bioimaging. Herein, a novel two-photon excited fluorescence dye has been synthesized. This V-shaped dye exhibited large two-photon excited fluorescence action cross-sections and high plasma membrane permeability. Cell imaging experiments demonstrated that the dye could stain living cells with bright two-photon excited fluorescence. All the results have indicated the potential of the dye as a basic platform for the development of two-photon excited fluorescence probes.

AB - The development of two-photon fluorophores remains an important issue. Dyes that possess both large two-photon excited fluorescence action cross-sections and cell membrane permeability are especially in demand to maximize the underlying virtue of two-photon microscopy for bioimaging. Herein, a novel two-photon excited fluorescence dye has been synthesized. This V-shaped dye exhibited large two-photon excited fluorescence action cross-sections and high plasma membrane permeability. Cell imaging experiments demonstrated that the dye could stain living cells with bright two-photon excited fluorescence. All the results have indicated the potential of the dye as a basic platform for the development of two-photon excited fluorescence probes.

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M3 - Article

AN - SCOPUS:84946594687

SN - 2050-7518

VL - 3

SP - 8644

EP - 8649

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 44

ER -

A membrane-permeable dye for living cells with large two-photon excited fluorescence action cross-sections for bioimaging

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