A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes

Zewen Wei; Huang Huang; Zicai Liang; Zhihong Li

doi:10.1109/MEMSYS.2010.5442365

A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes

Zewen Wei^*
, Huang Huang
, Zicai Liang
, Zhihong Li

^*Corresponding author for this work

Peking University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

Electroporation, in which electric pulses are applied to create transient pores in the cell membrane, is widely used to introduce biologically active molecules into cells. Here we report a novel electroporation chip with great performance and good compatibility with the standard multi-well plate used in biological research. We demonstrated a prototype chip integrating with a 12-well plate and successfully electroporated both adherent and supernatant cells. Using the standard expression cell line HEK-293 and expression vector pEGFP-C3, we realized excellent cell viability (80%) and transfection rate (90%), both much higher than those in previously reported methods.

Original language	English
Title of host publication	MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
Pages	951-954
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2010.5442365
Publication status	Published - 2010
Externally published	Yes
Event	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China Duration: 24 Jan 2010 → 28 Jan 2010

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
Country/Territory	China
City	Hong Kong
Period	24/01/10 → 28/01/10

Access to Document

10.1109/MEMSYS.2010.5442365

Cite this

Wei, Z., Huang, H., Liang, Z., & Li, Z. (2010). A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest (pp. 951-954). Article 5442365 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442365

Wei, Zewen ; Huang, Huang ; Liang, Zicai et al. / A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes. MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. pp. 951-954 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

@inproceedings{7b910fcafc4e436bac84310afa00fa11,

title = "A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes",

abstract = "Electroporation, in which electric pulses are applied to create transient pores in the cell membrane, is widely used to introduce biologically active molecules into cells. Here we report a novel electroporation chip with great performance and good compatibility with the standard multi-well plate used in biological research. We demonstrated a prototype chip integrating with a 12-well plate and successfully electroporated both adherent and supernatant cells. Using the standard expression cell line HEK-293 and expression vector pEGFP-C3, we realized excellent cell viability (80\%) and transfection rate (90\%), both much higher than those in previously reported methods.",

author = "Zewen Wei and Huang Huang and Zicai Liang and Zhihong Li",

year = "2010",

doi = "10.1109/MEMSYS.2010.5442365",

language = "English",

isbn = "9781424457649",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "951--954",

booktitle = "MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest",

note = "23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 ; Conference date: 24-01-2010 Through 28-01-2010",

}

Wei, Z, Huang, H, Liang, Z & Li, Z 2010, A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes. in MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest., 5442365, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 951-954, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 24/01/10. https://doi.org/10.1109/MEMSYS.2010.5442365

A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes. / Wei, Zewen; Huang, Huang; Liang, Zicai et al.
MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 951-954 5442365 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes

AU - Wei, Zewen

AU - Huang, Huang

AU - Liang, Zicai

AU - Li, Zhihong

PY - 2010

Y1 - 2010

N2 - Electroporation, in which electric pulses are applied to create transient pores in the cell membrane, is widely used to introduce biologically active molecules into cells. Here we report a novel electroporation chip with great performance and good compatibility with the standard multi-well plate used in biological research. We demonstrated a prototype chip integrating with a 12-well plate and successfully electroporated both adherent and supernatant cells. Using the standard expression cell line HEK-293 and expression vector pEGFP-C3, we realized excellent cell viability (80%) and transfection rate (90%), both much higher than those in previously reported methods.

AB - Electroporation, in which electric pulses are applied to create transient pores in the cell membrane, is widely used to introduce biologically active molecules into cells. Here we report a novel electroporation chip with great performance and good compatibility with the standard multi-well plate used in biological research. We demonstrated a prototype chip integrating with a 12-well plate and successfully electroporated both adherent and supernatant cells. Using the standard expression cell line HEK-293 and expression vector pEGFP-C3, we realized excellent cell viability (80%) and transfection rate (90%), both much higher than those in previously reported methods.

UR - https://www.scopus.com/pages/publications/77952772389

U2 - 10.1109/MEMSYS.2010.5442365

DO - 10.1109/MEMSYS.2010.5442365

M3 - Conference contribution

AN - SCOPUS:77952772389

SN - 9781424457649

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 951

EP - 954

BT - MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest

T2 - 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010

Y2 - 24 January 2010 through 28 January 2010

ER -

Wei Z, Huang H, Liang Z, Li Z. A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 951-954. 5442365. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2010.5442365

A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes

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