A continuous flow microfluidic-MS system for efficient OBOC screening

Weizhi Wang; Zewen Wei; Zihua Wang; Huailei Ma; Xiangli Bu; Zhiyuan Hu

doi:10.1039/c4ra12911c

A continuous flow microfluidic-MS system for efficient OBOC screening

Weizhi Wang
, Zewen Wei
, Zihua Wang
, Huailei Ma
, Xiangli Bu
, Zhiyuan Hu^*

^*Corresponding author for this work

National Center for Nanoscience and Technology
Academy of Military Medical Science China

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A microfluidic chip based method utilized for effective screening of high-throughput peptide libraries was achieved. Continuous flow bead trapping, sorting, arraying and in situ sequencing was integrated. Peptide library screening with 10⁵ beads was processed within 4 hours and peptide ligands toward the target protein AHA and APN were successfully discovered.

Original language	English
Pages (from-to)	61767-61770
Number of pages	4
Journal	RSC Advances
Volume	4
Issue number	106
DOIs	https://doi.org/10.1039/c4ra12911c
Publication status	Published - 2014
Externally published	Yes

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10.1039/c4ra12911c

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title = "A continuous flow microfluidic-MS system for efficient OBOC screening",

abstract = "A microfluidic chip based method utilized for effective screening of high-throughput peptide libraries was achieved. Continuous flow bead trapping, sorting, arraying and in situ sequencing was integrated. Peptide library screening with 105 beads was processed within 4 hours and peptide ligands toward the target protein AHA and APN were successfully discovered.",

author = "Weizhi Wang and Zewen Wei and Zihua Wang and Huailei Ma and Xiangli Bu and Zhiyuan Hu",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2014.",

year = "2014",

doi = "10.1039/c4ra12911c",

language = "English",

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T1 - A continuous flow microfluidic-MS system for efficient OBOC screening

AU - Wang, Weizhi

AU - Wei, Zewen

AU - Wang, Zihua

AU - Ma, Huailei

AU - Bu, Xiangli

AU - Hu, Zhiyuan

PY - 2014

Y1 - 2014

N2 - A microfluidic chip based method utilized for effective screening of high-throughput peptide libraries was achieved. Continuous flow bead trapping, sorting, arraying and in situ sequencing was integrated. Peptide library screening with 105 beads was processed within 4 hours and peptide ligands toward the target protein AHA and APN were successfully discovered.

AB - A microfluidic chip based method utilized for effective screening of high-throughput peptide libraries was achieved. Continuous flow bead trapping, sorting, arraying and in situ sequencing was integrated. Peptide library screening with 105 beads was processed within 4 hours and peptide ligands toward the target protein AHA and APN were successfully discovered.

UR - https://www.scopus.com/pages/publications/84912020122

U2 - 10.1039/c4ra12911c

DO - 10.1039/c4ra12911c

M3 - Article

AN - SCOPUS:84912020122

SN - 2046-2069

VL - 4

SP - 61767

EP - 61770

JO - RSC Advances

JF - RSC Advances

IS - 106

ER -

A continuous flow microfluidic-MS system for efficient OBOC screening

Abstract

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