毛细管区带电泳技术研究龙血竭有效单体与核酸适配体及凝血酶之间的相互作用

Yu Juan Li; Xin Xin Sun; Pan Pan Li; Wan Lu Ou; Feng Qu

doi:10.15918/j.tbit1001-0645.2019.01.018

毛细管区带电泳技术研究龙血竭有效单体与核酸适配体及凝血酶之间的相互作用

Translated title of the contribution: Interactions Between Aptamer, Bovine Thrombin and Active Molecules in Dragon's Blood by CZE

Yu Juan Li
, Xin Xin Sun^*
, Pan Pan Li
, Wan Lu Ou
, Feng Qu

^*Corresponding author for this work

School of Life Science

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Interactions between Apt29, bovine thrombin (B-Thr) and five active molecules in Dragon's blood were investigated based on capillary zone electrophoresis (CZE). The CZE conditions were set as following: effective capillary length of 40 cm, injection volume of 3.45 kPa, injection time of 5 s, voltage of +15 kV, temperature of 15℃, detection wavelength of 214 nm and 330 nm. The results show that K_b between Loureirin A and B-Thr is 4.73×10⁴ L/mol, and Loureirin B, Loureirin C, Resveratrol 7, 4-dihydroxyflavanone are no bound with B-Thr obviously. The K_b between Loureirin B, DHF and Apt29 are 1.98×10⁴, 1.83×10⁴ L/mol, respectively. Loureirin A, Loureirin C and Resveratrol are no bound with Apt29 obviously. The study indicates the interactions between B-Thr, Apt29 and active molecules in Dragon's blood. It can provide a basis for speculating the potential binding site of dragon's blood with thrombin, and delivering Dragon's blood to thrombin with aptamer as target delivery tool.

Translated title of the contribution	Interactions Between Aptamer, Bovine Thrombin and Active Molecules in Dragon's Blood by CZE
Original language	Chinese (Traditional)
Pages (from-to)	106-110
Number of pages	5
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	39
Issue number	1
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2019.01.018
Publication status	Published - 1 Jan 2019

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10.15918/j.tbit1001-0645.2019.01.018

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title = "毛细管区带电泳技术研究龙血竭有效单体与核酸适配体及凝血酶之间的相互作用",

abstract = "Interactions between Apt29, bovine thrombin (B-Thr) and five active molecules in Dragon's blood were investigated based on capillary zone electrophoresis (CZE). The CZE conditions were set as following: effective capillary length of 40 cm, injection volume of 3.45 kPa, injection time of 5 s, voltage of +15 kV, temperature of 15℃, detection wavelength of 214 nm and 330 nm. The results show that Kb between Loureirin A and B-Thr is 4.73{\texttimes}104 L/mol, and Loureirin B, Loureirin C, Resveratrol 7, 4-dihydroxyflavanone are no bound with B-Thr obviously. The Kb between Loureirin B, DHF and Apt29 are 1.98{\texttimes}104, 1.83{\texttimes}104 L/mol, respectively. Loureirin A, Loureirin C and Resveratrol are no bound with Apt29 obviously. The study indicates the interactions between B-Thr, Apt29 and active molecules in Dragon's blood. It can provide a basis for speculating the potential binding site of dragon's blood with thrombin, and delivering Dragon's blood to thrombin with aptamer as target delivery tool.",

keywords = "Active molecules, Aptamer, B-Thr, Capillary zone electrophoresis, Dragon's blood",

author = "Li, \{Yu Juan\} and Sun, \{Xin Xin\} and Li, \{Pan Pan\} and Ou, \{Wan Lu\} and Feng Qu",

year = "2019",

month = jan,

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doi = "10.15918/j.tbit1001-0645.2019.01.018",

language = "繁体中文",

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number = "1",

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T1 - 毛细管区带电泳技术研究龙血竭有效单体与核酸适配体及凝血酶之间的相互作用

AU - Li, Yu Juan

AU - Sun, Xin Xin

AU - Li, Pan Pan

AU - Ou, Wan Lu

AU - Qu, Feng

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Interactions between Apt29, bovine thrombin (B-Thr) and five active molecules in Dragon's blood were investigated based on capillary zone electrophoresis (CZE). The CZE conditions were set as following: effective capillary length of 40 cm, injection volume of 3.45 kPa, injection time of 5 s, voltage of +15 kV, temperature of 15℃, detection wavelength of 214 nm and 330 nm. The results show that Kb between Loureirin A and B-Thr is 4.73×104 L/mol, and Loureirin B, Loureirin C, Resveratrol 7, 4-dihydroxyflavanone are no bound with B-Thr obviously. The Kb between Loureirin B, DHF and Apt29 are 1.98×104, 1.83×104 L/mol, respectively. Loureirin A, Loureirin C and Resveratrol are no bound with Apt29 obviously. The study indicates the interactions between B-Thr, Apt29 and active molecules in Dragon's blood. It can provide a basis for speculating the potential binding site of dragon's blood with thrombin, and delivering Dragon's blood to thrombin with aptamer as target delivery tool.

AB - Interactions between Apt29, bovine thrombin (B-Thr) and five active molecules in Dragon's blood were investigated based on capillary zone electrophoresis (CZE). The CZE conditions were set as following: effective capillary length of 40 cm, injection volume of 3.45 kPa, injection time of 5 s, voltage of +15 kV, temperature of 15℃, detection wavelength of 214 nm and 330 nm. The results show that Kb between Loureirin A and B-Thr is 4.73×104 L/mol, and Loureirin B, Loureirin C, Resveratrol 7, 4-dihydroxyflavanone are no bound with B-Thr obviously. The Kb between Loureirin B, DHF and Apt29 are 1.98×104, 1.83×104 L/mol, respectively. Loureirin A, Loureirin C and Resveratrol are no bound with Apt29 obviously. The study indicates the interactions between B-Thr, Apt29 and active molecules in Dragon's blood. It can provide a basis for speculating the potential binding site of dragon's blood with thrombin, and delivering Dragon's blood to thrombin with aptamer as target delivery tool.

KW - Active molecules

KW - Aptamer

KW - B-Thr

KW - Capillary zone electrophoresis

KW - Dragon's blood

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DO - 10.15918/j.tbit1001-0645.2019.01.018

M3 - 文章

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SN - 1001-0645

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JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

IS - 1

ER -

毛细管区带电泳技术研究龙血竭有效单体与核酸适配体及凝血酶之间的相互作用

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