一种低密度脂蛋白受体相关蛋白在凡纳滨对虾(Litopenaeus vannamei)抗弧菌感染中的功能研究

Low-density lipoprotein receptor-related protein (LRP) is a cell surface protein and an endocytic and cell signaling receptor. Its role in regulating blood lipid balance in vertebrates has been well known. Previously, one LRP gene was reported to have a significant difference in expression between the resistant and susceptible families to Vibrio in Litopenaeus vannamei by comparative transcriptome analysis. We cloned the full-length cDNA of the gene and analyzed its expression characteristics, and carried out a preliminary study on its function in order to clarify the role of the gene in L. vannamei anti-vibrio infection. Results show that there were two transcripts of this gene in L. vannamei, named LvLRP1-1 and LvLRP1-2. The length of LvLRP1-1 is 916 bp encoding 226 amino acids, while the length of LvLRP1-2 is 787 bp encoding 183 amino acids. The cDNA sequences of the two transcripts are highly consistent, except for the only difference that the cDNA of LvLRP1-1 contained extra 129 bp that encodes 43 more amino acids than LvLRP1-2. Tissue expression analysis showed that LvLRP1-1 was highly expressed in the eye stalk, epidermis, brain and heart, while LvLRP1-2 was highly expressed only in the eye stalk and epidermis. The change in the transcriptional expression of LvLRP1-1 and LvLRP1-2 after Vibrio parahaemolyticus infection was consistent, and the gene expression was significantly increased at 3 hours after infection, and returned to the normal level after 6 hours. The load of Vibrio and mortality rate after Vibrio infection were significantly increased by simultaneously interfering LvLRP1-1 and LvLRP1-2 with double-stranded RNA. Therefore, LRP1 plays an important role in the resistance to Vibrio infection in L. vannamei.