μ CP- and DLP-Based 3D Bioprinting for Liver Tumor Microenvironment Construction

The in vitro reconstruction of an authentic liver tumor microenvironment holds significant promise for drug screening and clinical research. However, current 3D tumor models, such as those cultured in U96-well plates or via bioprinting, exhibit limitations in recapitulating dynamic cell-matrix interactions. This study presents a stepwise fabrication approach: liver tumor cells are first cultured via microcontact printing to form spheroids, followed by digital micromirror device-based microfluidic channel photopolymerization to encapsulate the spheroids within a tumor-stromal compartment, thereby constructing a physiologically relevant liver tumor microenvironment. This model enables systematic investigation of tumor-stroma crosstalk and high-throughput anticancer drug screening, providing a controllable in vitro platform for precision oncology and metastatic mechanism analysis.