α-Synuclein binds to cytoplasmic vesicles in U251 glioblastoma cells

Jinyan Duan; Zhengxin Ying; Yang Su; Fankai Lin; Yulin Deng

doi:10.1016/j.neulet.2017.01.067

α-Synuclein binds to cytoplasmic vesicles in U251 glioblastoma cells

Jinyan Duan, Zhengxin Ying, Yang Su, Fankai Lin, Yulin Deng^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

α-Synuclein is the major component of Lewy bodies, Lewy neurites, and glial cytoplasmic inclusions. It plays an important role in neurodegenerative diseases such as Parkinson's disease, multiple system atrophy, and other synucleinopathies. However, the pathogenesis and neurodegenerative effects of α-synuclein remain unknown. In this study, we established an α-synuclein and an α-synuclein-EGFP overexpressing U251 cell line. α-Synuclein overexpression increases oxidative stress and alters the cell surface and mitochondrial morphologies. We provide fluorescent-protein tagging, immunofluorescence and ultrastructural evidence showing that α-synuclein accumulations are associated with clusters of cytoplasmic vesicles and the diameter of these vesicles increases by H₂O₂ in a time- and dose-dependent manner.

Original language	English
Pages (from-to)	148-152
Number of pages	5
Journal	Neuroscience Letters
Volume	642
DOIs	https://doi.org/10.1016/j.neulet.2017.01.067
Publication status	Published - 6 Mar 2017

Keywords

Cytoplasmic vesicles
Oxidative stress
α-synuclein

Access to Document

10.1016/j.neulet.2017.01.067

Cite this

@article{7e1a085c38f14d7aafa8c496e5ea53d4,

title = "α-Synuclein binds to cytoplasmic vesicles in U251 glioblastoma cells",

abstract = "α-Synuclein is the major component of Lewy bodies, Lewy neurites, and glial cytoplasmic inclusions. It plays an important role in neurodegenerative diseases such as Parkinson's disease, multiple system atrophy, and other synucleinopathies. However, the pathogenesis and neurodegenerative effects of α-synuclein remain unknown. In this study, we established an α-synuclein and an α-synuclein-EGFP overexpressing U251 cell line. α-Synuclein overexpression increases oxidative stress and alters the cell surface and mitochondrial morphologies. We provide fluorescent-protein tagging, immunofluorescence and ultrastructural evidence showing that α-synuclein accumulations are associated with clusters of cytoplasmic vesicles and the diameter of these vesicles increases by H2O2 in a time- and dose-dependent manner.",

keywords = "Cytoplasmic vesicles, Oxidative stress, α-synuclein",

author = "Jinyan Duan and Zhengxin Ying and Yang Su and Fankai Lin and Yulin Deng",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

year = "2017",

month = mar,

day = "6",

doi = "10.1016/j.neulet.2017.01.067",

language = "English",

volume = "642",

pages = "148--152",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - α-Synuclein binds to cytoplasmic vesicles in U251 glioblastoma cells

AU - Duan, Jinyan

AU - Ying, Zhengxin

AU - Su, Yang

AU - Lin, Fankai

AU - Deng, Yulin

PY - 2017/3/6

Y1 - 2017/3/6

N2 - α-Synuclein is the major component of Lewy bodies, Lewy neurites, and glial cytoplasmic inclusions. It plays an important role in neurodegenerative diseases such as Parkinson's disease, multiple system atrophy, and other synucleinopathies. However, the pathogenesis and neurodegenerative effects of α-synuclein remain unknown. In this study, we established an α-synuclein and an α-synuclein-EGFP overexpressing U251 cell line. α-Synuclein overexpression increases oxidative stress and alters the cell surface and mitochondrial morphologies. We provide fluorescent-protein tagging, immunofluorescence and ultrastructural evidence showing that α-synuclein accumulations are associated with clusters of cytoplasmic vesicles and the diameter of these vesicles increases by H2O2 in a time- and dose-dependent manner.

AB - α-Synuclein is the major component of Lewy bodies, Lewy neurites, and glial cytoplasmic inclusions. It plays an important role in neurodegenerative diseases such as Parkinson's disease, multiple system atrophy, and other synucleinopathies. However, the pathogenesis and neurodegenerative effects of α-synuclein remain unknown. In this study, we established an α-synuclein and an α-synuclein-EGFP overexpressing U251 cell line. α-Synuclein overexpression increases oxidative stress and alters the cell surface and mitochondrial morphologies. We provide fluorescent-protein tagging, immunofluorescence and ultrastructural evidence showing that α-synuclein accumulations are associated with clusters of cytoplasmic vesicles and the diameter of these vesicles increases by H2O2 in a time- and dose-dependent manner.

KW - Cytoplasmic vesicles

KW - Oxidative stress

KW - α-synuclein

UR - http://www.scopus.com/inward/record.url?scp=85012117571&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2017.01.067

DO - 10.1016/j.neulet.2017.01.067

M3 - Article

C2 - 28159635

AN - SCOPUS:85012117571

SN - 0304-3940

VL - 642

SP - 148

EP - 152

JO - Neuroscience Letters

JF - Neuroscience Letters

ER -

α-Synuclein binds to cytoplasmic vesicles in U251 glioblastoma cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this